5α‐reductase activity in epithelium and stroma of prostates from intact and castrated dogs treated with androstenedione, the aromatase inhibitor 1‐methyl‐1,4‐androstadiene‐3, 17‐dione, and cyproterone acetate

• Sabine Tunn

• Holger Hochstrate

• Ursula‐F. Habenicht

• Michael Krieg

抄録

In addition to the histology of epithelial and stromal elements of prostates from intact dogs (group 0) and castrated dogs (group I), the latter of which were treated with androstenedione (group II), androstenedione plus the aromatase inhibitor 1‐methyl‐1,4‐androstadiene‐3,17‐dione (group III), or androstenedione plus aromatase inhibitor and cyproterone acetate (group IV) (Habenicht and El Etreby: The Prostate 11:133–143, 1987) it was of interest to study the influence of such in vivo treatment on the prostatic 5α‐reductase, which is responsible for the cellular conversion of testosterone to 5α‐dihydrotestosterone. Michaelis constants (K M ) and maximal activities (V max ) of 5α‐reductase were determined under optimized incubation conditions in mechanically separated epithelium and stroma. The metabolites were separated by high‐performance liquid chromatography and determined radiometrically. The main results were: 1) The mean K M (nM ± SEM) was significantly ( P < .001) higher in epithelium (892 ± 132) than stroma (70 ± 11). The same was true concerning the V max (pmol · mg protein −1 · h −1 · SEM) in epithelium (54.6 ± 5.8) as compared to stroma (13.0 ± 2.0). 2) No specific in vivo or in vitro effect of the aromatase inhibitor on the K M and V max data was found. 3) In prostates of intact dogs and dogs of group II the proportion of epithelial 5α‐reductase exceeded distinctly that of stromal 5α‐reductase. 4) In groups I, III, and IV the proportion of epithelial 5α‐reductase was rather low. These data were discussed in the light of the histological findings.

収録刊行物

• The Prostate

  The Prostate 12 (3), 243-253, 1988-01

  Wiley

キーワード

• Urology
• Oncology

詳細情報

• CRID: 1360855569897138176
• NII論文ID: 30025839375
• DOI: 10.1002/pros.2990120307
• ISSN: 10970045
• ISSN: 02704137
• ISSN: http://id.crossref.org/issn/02704137
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fpros.2990120307
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1002/pros.2990120307
• データソース種別:
  • Crossref
  • CiNii Articles