Tobacco Transcription Factor WRKY1 Is Phosphorylated by the MAP Kinase SIPK and Mediates HR-Like Cell Death in Tobacco

• Frank L. H. Menke

• Hong-Gu Kang

• Zhixiang Chen

• Jeong Mee Park

• Dhirendra Kumar

• Daniel F. Klessig

抄録

The salicylic acid-induced protein kinase (SIPK) of tobacco, which is a mitogen-activated protein kinase (MAPK), is activated by various biotic and abiotic treatments. Overexpression of SIPK has been shown to trigger cell death. In this study, a targeted yeast two-hybrid approach identified the tobacco transcription factor WRKY1 as a potential substrate. SIPK phosphorylated WRKY1, which resulted in enhanced DNA-binding activity of WRKY1 to its cognate binding site, a W box sequence from the tobacco chitinase gene CHN50. SIPK-mediated enhancement of WRKY1 DNA-binding activity was inhibited by staurosporine, a general kinase inhibitor. Co-expression of SIPK and WRKY1 in Nicotiana benthamiana led to more rapid cell death than expression of SIPK alone, suggesting that WRKY1 is involved in the formation of hypersensitive response-like cell death and may be a component of the signaling cascade downstream of SIPK.

収録刊行物

• Molecular Plant-Microbe Interactions®

  Molecular Plant-Microbe Interactions® 18 (10), 1027-1034, 2005-10

  Scientific Societies

キーワード

• Agronomy and Crop Science
• General Medicine
• Physiology

詳細情報

• CRID: 1363670318220335232
• NII論文ID: 30027633761
• DOI: 10.1094/mpmi-18-1027
• ISSN: 19437706
• ISSN: 08940282
• ISSN: http://id.crossref.org/issn/08940282
• Web Site: https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-18-1027
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