Leukocyte Removal Filter‐Passed Lymphocytes Produce Large Amounts of Interleukin‐4 in Immunotherapy for Inflammatory Bowel Disease: Role of Bystander Suppression

• Mitsunori Noguchi

• Nobuo Hiwatashi

• Tomohiko Hayakawa

• Takayoshi Toyota

Abstract

Abstract: To determine immunosuppression by leukocy‐tapheresis, we studied the immune profiles of filter‐passed lymphocytes (FPLs) in patients with ulcerative colitis. These patients were treated with a leukocytapheresis (LCAP) filter of granulocyte apheresis (GCAP) column. Cytokine profiles or FPLs and peripheral blood mononuclear cells (PBMCs) were examined using interleukin (IL)‐4 and interferon‐gamma (IFN‐7) immunoassay with lectin stimulation. The IL‐4 production of LCAP FPLs was significantly higher than in prefilter PBMCs and GCAP column‐passed lymphocytes (median: 540 pg/ml 10 6 cells versus 4.7 and 2.9, respectively, p = 0.001). IL‐4 production in PBMCs was increased after LCAP. IFN‐γ production was not increased in the FPLs. The IL‐4 immune deviation was not shown in the patients treated with GCAP. LCAP FPLs showed increased CD4+DR‐ cells and decreases CD4+DR+ cells in comparison to PBMCs. LCAP FPLs could produce IL‐4 and may lead to bystander suppression.

Journal

• Therapeutic Apheresis

  Therapeutic Apheresis 2 (2), 109-114, 1998-05

  Wiley

Keywords

• General Medicine

Details

• CRID: 1360292618755286016
• NII Article ID: 30030279388
• DOI: 10.1111/j.1744-9987.1998.tb00086.x
• ISSN: 15260968
• ISSN: 10916660
• ISSN: http://id.crossref.org/issn/10916660
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1744-9987.1998.tb00086.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1744-9987.1998.tb00086.x
• Data Source:
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