Mapping of Four Simple Sequence Repeat(SSR) Markers on Rat Chromosome 4.

• MASHIMO Tomoji

  Graduate School of Human and Environmental Studies, Kyoto University Department of Laboratory Medicine, Shimane Medical University

• NABIKA Toru

  Department of Laboratory Medicine, Shimane Medical University

• KITADA Kazuhiro

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

• SERIKAWA Tadao

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

• MATSUMOTO Chiho

  Graduate School of Human and Environmental Studies, Kyoto University

• IKEDA Katsumi

  Department of International Preventive Nutritional Medicine, Kyoto University

• NARA Yasuo

  Graduate School of Integrated Science and Art, University of East Asia

• MASUDA Junichi

  Department of Laboratory Medicine, Shimane Medical University

• YAMORI Yukio

  Graduate School of Human and Environmental Studies, Kyoto University

抄録

We previously reported that several markers on rat chromosome (Chr) 4 cosegregated with the occurrence of cerebral stroke and brain edema in stroke-prone spontaneously hypertensive rats (SHRSP). To obtain insights into the positional candidate genes for stroke susceptibility in this region, we mapped four genes, Taurine transporter (Tau), tumor necrosis factor receptor (Tnfr), GABA transporter (Gat1) and glucose transporter-3 (Glut3) genes, using newly developed simple sequence repeat (SSR) markers on rat Chr 4. We isolated the SSRs for the genes either by screening a rat genomic library or by searching the GenBank database. By linkage analysis using two sets of backcrosses, Gat1 and Tnfr were mapped in the region associated with stroke, while Taut was located distant from the region. The Glut3 locus was also assigned to rat Chr 4 using a rat x mouse hybrid clone panel. These results indicated that the Tnfr, Gat1 and Glut3 genes were good positional candidates for the stroke susceptibility in SHRSP, suggesting that further evaluation of these genes by functional studies could prove useful. (Hypertens Res 2000; 23: 47-50)

収録刊行物

• Hypertension Research

  Hypertension Research 23 (1), 47-50, 2000

  日本高血圧学会

キーワード

• stroke susceptibility
• stroke-prone spontaneously hypertensive rat
• candidate genes
• simple sequence repeat markers
• comparative map

詳細情報

• CRID: 1390001204720180096
• NII論文ID: 130003456417
• NII論文ID: 30034931878
• NII書誌ID: AA10847079
• DOI: 10.1291/hypres.23.47
• COI: 1:CAS:528:DC%2BD3cXht1ans7k%3D
• ISSN: 13484214
• ISSN: 09169636
• ISSN: http://id.crossref.org/issn/09169636
• PubMed: 10737135
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可