Growth of noninfected and <i>Plasmodiophora brassicae</i> infected cabbage callus in culture

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<jats:p> Noninfected cabbage callus and Plasmodiophora brassicae infected callus isolated from clubroot galls were maintained for over 40 successive transfers on Murashige-Skoog's medium modified to contain 0.5 mg/l of α-naphthalene acetic acid. Infected callus if transferred at 7- to 12-day intervals grew rapidly, doubling its dry weight about every 3 days, whereas noninfected callus doubled its dry weight about every 5 days. Approximately one-third of the cells in infected callus contained the parasite in various stages of its life cycle, ranging from small vegetative plasmodia to mature resting sporangia. Sporangia isolated from callus were viable and produced clubroot inoculated on cabbage seedlings. When a high percentage of the plasmodia in infected cells in any portion of a callus underwent sporogenesis, the callus growth slowed and the tissues became brown. By transferring only actively proliferating callus a high percentage of plasmodia could be maintained in the vegetative condition. Infected callus resembles closely gall tissue from natural clubroot both cytologically and chemically and thus should be a useful material for studying parasitism and the processes of hypertrophy and hyperplasia in a contaminant-free system. </jats:p>

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