高速液体クロマトグラフィーによる鶏組織中に残留する17種の合成抗菌剤の同時分析法

DOI Web Site 被引用文献1件

書誌事項

タイトル別名
  • Simultaneous Determination of 17 Antibacterials in Chicken Tissues by High Performance Liquid Chromatography
  • コウソク エキタイ クロマトグラフィー ニ ヨル ニワトリ ソシキチュウ ニ

この論文をさがす

抄録

A high performance liquid chromatographic method (HPLC) for determination of 7 sulfonamides, clopidol, trimethoprim, ormethoprim, furazolidone, dinitolmide, pyrimethamine, ethopabate, difurazone, nicarbazin, and decoquinate, in chicken tissues was developed.<br>The drugs were extracted from tissues by homogenizing with warm methanol. The homogenate was centrifuged and the supernatant was evaporated to dryness. The residue was dissolved in buffer solution (pH 5.5) and partitioned with n-hexane. The buffer solution was then partitioned with chloroform. The chloroform layer was evaporated to dryness and the residue was dissolved in 85% acetonitrile and applied to an alumina column. The drugs were eluted with 85% acetonitrile and the eluate was applied to an HPLC column packed with Nucleosil 5C18.<br>Decoquinate was eluted with methanol-water (9:1; detected at 315nm), difurazone and nicarbazine with acetonitrile-acetic acid-water (50:0.5:50; detected at 350nm), and the other drugs with acetonitrile-acetic acid-water (14:0.5:86; detected at 270nm and at 350nm).<br>The average recoveries of all drugs added at the level of 0.2ppm were more than 65.5%. The detection limits of clopidol, difurazone, nicarbazin, sulfamerazine and sulfamethoxy-pyridazine were 0.03ppm, and those of the other drugs were 0.05ppm.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 29 (1), 13-20_1, 1988

    公益社団法人 日本食品衛生学会

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ