Protease inhibitors in the haemolymph of the silkworm, <i>Bombyx mori</i>

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  • 家蚕血液中のプロテアーゼ阻害剤　家蚕消化液および中腸のプロテアーゼに対する作用
  • 家蚕血液中のプロテアーゼインヒビター : 家蚕消化液および中腸のプロテアーゼに対する作用
  • カサン ケツエキチュウ ノ プロテアーゼ インヒビター カサン ショウカエキ
  • 家蚕消化液および中腸のプロテアーゼに対する作用

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Abstract

The effect of inhibitors in the haemolymph of the silkworm on the proteases from the digestive fluid and midgut of the silkworm was studied and compared with their effect on trypsin and chymotrypsin.<br>Protease activity of the midgut and the digestive fluid was inhibited by the addition of haemolymph. The enzyme activity-haemolymph concentration curve of digestive fluid protease was similar to that of trypsin, but curve of midgut protease was somewhat different from the above proteases.<br>Three inhibitors were demonstrated by the gel filtration of haemolymph on Sephadex G-75; the first fraction inhibited the digestive fluid and midgut proteases, the third one was effective to trypsin and chymotrypsin and the second one inhibited all the four proteases. The first and third fractions were heat stable but the second one was heat labile.<br>The inhibitory effect decreased during the fifth instar, increased just before pupation and there was no great change in the pupal stage.

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