Chemiluminescence Detection of Thiols by High-Performance Liquid Chromatography Using o-Phthalaldehyde and N-(4-Aminobutyl)-N-ethylisoluminol as Precolumn Derivatization Reagents.
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- SANO Akira
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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- NAKAMURA Hiroshi
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
書誌事項
- タイトル別名
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- Chemiluminescence Detection of Thiols b
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A method for the determination of thiols by high-performance liquid chromatography (HPLC) with chemiluminescence detection has been developed. The method is based on the precolumn derivatization of thiols with o-phthalaldehyde and N-(4-aminobutyl)-N-ethylisoluminol to form isoindole derivatives and the reversed-phase HPLC separation of the derivatives, followed by successive postcolumn chemiluminescence reactions with hydrogen peroxide and hematin. The limits of the detection of glutathione (GSH) and cysteine were 1.5 nM (15 fmol/injection) and 3.5 nM (35 fmol/injection), respectively. The calibration curve for GSH was linear in the range 5 - 1000 nM (1 - 200 pmol/200 μl). The method was applied to the determination of GSH in human blood. The recovery of GSH added to the blood at the concentration level of 500 μM was about 70%. The GSH concentrations in the blood of three healthy male persons were 502 - 770 μM.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 14 (4), 731-735, 1998
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204255813632
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- NII論文ID
- 130004439890
- 40005335288
- 10002416406
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DyaK1cXltlCksbw%3D
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- ISSN
- 13482246
- 09106340
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I4547632
- https://ndlsearch.ndl.go.jp/books/R000000004-I4538510
- https://link.springer.com/content/pdf/10.2116/analsci.14.731.pdf
- https://link.springer.com/article/10.2116/analsci.14.731/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/14/4/14_4_731/_pdf
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