Sequential Injection Analysis with Chemiluminescence Detection for the Antioxidative Activity against Singlet Oxygen

• MIYAMOTO Aoi

  Graduate School of Biomedical Sciences, Nagasaki University

• NAKAMURA Kuniko

  Graduate School of Biomedical Sciences, Nagasaki University

• OHBA Yoshihito

  Graduate School of Biomedical Sciences, Nagasaki University

• KISHIKAWA Naoya

  Graduate School of Biomedical Sciences, Nagasaki University

• NAKASHIMA Kenichiro

  Graduate School of Biomedical Sciences, Nagasaki University

• KURODA Naotaka

  Graduate School of Biomedical Sciences, Nagasaki University

抄録

A sequential injection analysis (SIA) with chemiluminescence (CL) detection was developed for the measurement of antioxidative activity against singlet oxygen (¹O₂). Lactoperoxidase-hydrogen peroxide-bromide ion system was used for the generation of ¹O₂. When a 100 mM sodium acetate buffer (pH 4.5) was used as a carrier solution, the SIA-CL system could be optimized with respect to the flow-rate of the carrier, concentration of reagents and their aspiration order. The antioxidative activity was expressed as an attenuation of luminol CL due to the quenching of ¹O₂ by an antioxidant. The relative standard deviations of antioxidative activity (n = 3) against ¹O₂ for within- and between-day analyses were ≤ 1.6% (20 µM Trolox). The system was successfully applied to the assay of antioxidative activities of various antioxidants including vitamin supplements at a rate of 10 samples within 15 min. The proposed SIA-CL system was rapid and reproducible with minimum consumption of the sample and of reagents, and thus was useful for the screening of compounds possessing antioxidative activity against ¹O₂.

収録刊行物

• Analytical Sciences

  Analytical Sciences 22 (1), 73-76, 2006

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204255776256
• NII論文ID: 130004441148
• NII論文ID: 40007066567
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.22.73
• COI: 1:CAS:528:DC%2BD28XntVyitQ%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 7763396
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7763396
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.22.73.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.22.73/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/22/1/22_1_73/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006206165
• 本文言語コード: en
• データソース種別:
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  • NDL
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  • CiNii Articles
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