Distribution of PrPSc in Cattle with Bovine Spongiform Encephalopathy Slaughtered at Abattoirs in Japan

• Iwata Naoko

  Department of Pathology, National Institute of Infectious Diseases, Japan

• Sato Yuko

  Department of Pathology, National Institute of Infectious Diseases, Japan

• Higuchi Yoshimi

  Department of Pathology, National Institute of Infectious Diseases, Japan

• Nohtomi Kyoko

  Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Japan

• Nagata Noriyo

  Department of Pathology, National Institute of Infectious Diseases, Japan

• Hasegawa Hideki

  Department of Pathology, National Institute of Infectious Diseases, Japan

• Tobiume Minoru

  Department of Pathology, National Institute of Infectious Diseases, Japan

• Nakamura Yuko

  Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Japan

• Hagiwara Ken’ichi

  Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Japan

• Furuoka Hidefumi

  Department of Pathobiological Science, Obihiro University of Agriculture and Veterinary Medicine, Japan

• Horiuchi Motohiro

  Laboratory of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Japan

• Yamakawa Yoshio

  Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Japan

• Sata Tetsutaro

  Department of Pathology, National Institute of Infectious Diseases, Japan

書誌事項

タイトル別名

• Distribution of PrP^Sc in Cattle with Bovine Spongiform Encephalopathy Slaughtered at Abattoirs in Japan

抄録

Three 80- to 95-month-old Holstein dairy cattle infected naturally with the agent of bovine spongiform encephalopathy (BSE) and slaughtered at abattoirs in Japan were examined for the distribution of disease-specific and protease-resistant prion protein (PrP^Sc) by immunohistochemistry (IHC) and Western blot (WB) analyses. The cattle showed no clinical signs or symptoms relevant to BSE but were screened as positive by enzyme-linked immunosorbent assay, a rapid test for BSE. This positive result was confirmed by IHC or WB in a specimen of the medulla oblongata. Histopathologically, these cattle showed no vacuolation in tissue sections from the central nervous system except for the medulla oblongata. Both IHC and WB analyses revealed PrP^Sc accumulation in the brain, spinal cord, satellite and ganglionic cells of the dorsal root ganglia, and the myenteric plexus of the distal ileum. In addition, small amounts of PrP^Sc were detected in the peripheral nerves of 2 cattle by WB. No PrP^Sc was demonstrated by either method in the Peyer’s patches of the distal ileum; lymphoid tissues including the palatine tonsils, lymph nodes, and spleen; or other tissues. The distribution of PrP^Sc accumulation in the preclinical stage was different between naturally infected cattle and cattle inoculated experimentally with the BSE agent.

収録刊行物

• Japanese Journal of Infectious Diseases

  Japanese Journal of Infectious Diseases 59 (2), 100-107, 2006-04-28

  国立感染症研究所

キーワード

• Infectious Diseases
• Microbiology (medical)
• General Medicine

詳細情報

• CRID: 1390017511236814592
• NII論文ID: 40007227551
• NII書誌ID: AA1132885X
• DOI: 10.7883/yoken.jjid.2006.100
• ISSN: 18842836
• ISSN: 13446304
• ISSN: http://id.crossref.org/issn/13446304
• NDL書誌ID: 7890532
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7890532
• Web Site: https://www.jstage.jst.go.jp/article/yoken/59/2/59_JJID.2006.100/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006312831
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可