Merkel Cell-Nerve Cell Interaction Undergoes Formation of a Synapse-like Structure in a Primary Culture

• Shimohira-Yamasaki Mineko

  Department of Pathology & Biodefence, Faculty of Medicine, Saga University Division of Dermatology, Faculty of Medicine, Saga University

• Toda Shuji

  Department of Pathology & Biodefence, Faculty of Medicine, Saga University

• Narisawa Yutaka

  Division of Dermatology, Faculty of Medicine, Saga University

• Sugihara Hajime

  Department of Pathology & Biodefence, Faculty of Medicine, Saga University International University of Health and Welfare, The School of Rehabilitation Sciences

Abstract

Merkel cells have been assumed to guide nerve fibers to the skin. However, there has been little in vitro evidence that supports this hypothesis, because there is no suitable established culture system of Merkel cells. Here we show that Merkel cells isolated from rat footpad skin were successfully cultured in a monolayer with keratinocytes. Keratinocytes did not affect any structural changes in Merkel cells. When nerve cells (NG108-15 or PC12) were added to the culture system, both nerve fibers and cytoplasmic processes of Merkel cells outgrew and cooperatively organized synapse-like structures at their contact points. Nerve cells promoted Merkel cell survival, compared with keratinocytes only. Merkel cell proliferation was not detected in all conditions, even with nerve growth factor, neurotrophin-3, interleukin-6 and tumor necrosis factor-α. The data suggest, firstly, that Merkel cells may guide nerve fibers to the skin by interacting with nerve cells; and, secondly, that nerve cells, but not keratinocytes, may produce some survival factors other than the cytokines above for Merkel cells, although Merkel cells may be a terminally differentiated cell type. Our method could open a way to study Merkel cell biology.<br>

Journal

• Cell Structure and Function

  Cell Structure and Function 31 (1), 39-45, 2006

  Japan Society for Cell Biology

Keywords

• Merkel cells
• nerve cells
• cell-cell interaction
• synapse-like structure
• culture method

Details

• CRID: 1390001204694643200
• NII Article ID: 130004137505
• NII Article ID: 40015510798
• NII Book ID: AA0060007X
• DOI: 10.1247/csf.31.39
• ISSN: 13473700
• ISSN: 03867196
• NDL BIB ID: 8837746
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8837746
• Web Site: http://www.jstage.jst.go.jp/article/csf/31/1/31_1_39/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007217493
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed