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- Kasai Shinji
- Department of Medical Entomology, National Institute of Infectious Diseases, Japan
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- Komagata Osamu
- Department of Medical Entomology, National Institute of Infectious Diseases, Japan
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- Tomita Takashi
- Department of Medical Entomology, National Institute of Infectious Diseases, Japan
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- Sawabe Kyoko
- Department of Medical Entomology, National Institute of Infectious Diseases, Japan
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- Tsuda Yoshio
- Department of Medical Entomology, National Institute of Infectious Diseases, Japan
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- Kurahashi Hiromu
- Department of Medical Entomology, National Institute of Infectious Diseases, Japan
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- Ishikawa Takeshi
- Shell Company Co., Ltd., Japan
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- Motoki Mitsugu
- APEX Industry Co., Ltd., Japan
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- Takahashi Tomoya
- Fuji Environmental Service Co., Ltd., Japan
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- Tanikawa Tsutomu
- Ikari Corporation, Japan
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- Yoshida Masahiro
- Ikimono Kenkyuusha, Japan
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- Shinjo Goro
- Japan Environmental Sanitation Center, Japan
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- Hashimoto Tomoyuki
- Japan Environmental Sanitation Center, Japan
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- Higa Yukiko
- Center of International Collaborative Research, Nagasaki University, Japan
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- Kobayashi Mutsuo
- Department of Medical Entomology, National Institute of Infectious Diseases, Japan
書誌事項
- タイトル別名
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- PCR-Based Identification of <i>Culex pipiens</i> Complex Collected in Japan
この論文をさがす
抄録
<p>The Culex pipiens complex consists of vector mosquitoes that transmit important human pathogens. In this study we established a simplified method to distinguish three members of the Cx. pipiens complex, Cx. p. pallens Coquillet, Cx. p. form molestus Forskal, and Cx. quinquefasciatus Say, collected in Japan. Sequence analysis of the Drosophila Ace-orthologous acetylcholinesterase (Ace) gene (668 to 680 bp) revealed that a single polymorphic region characterizes each species. Based on this region, specific primers that distinguish Cx. p. form molestus (ACEpip2) and Cx. p. pallens (ACEpall2) were newly designed. Polymerase chain reactions were performed with the genomic DNA of Culex mosquitoes as the template, and these primers clearly distinguished two Culex spp. The accuracy of the designed primers was evaluated with 38 colonies of mosquito samples collected from 9 prefectures of Japan. The testing revealed that the distribution of anautogenous Cx. p. pipiens has not been confirmed in Japan. It also revealed that the male of Cx. p. pallens possesses an Ace gene haplotype that is highly similar to the sequence of Cx. quinquefasciatus. This improved method allows the evaluation of vector competence of Cx. p. form molestus, which is the suspected vector of West Nile virus.<tt> </tt></p>
収録刊行物
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 61 (3), 184-191, 2008-05-28
国立感染症研究所
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詳細情報 詳細情報について
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- CRID
- 1390858209198454016
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- NII論文ID
- 40016054642
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- NII書誌ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
- http://id.crossref.org/issn/13446304
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- NDL書誌ID
- 9508410
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可