Fluorescent Staining Analysis of Chromosomes in Pear (Pyrus spp.)
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- Yamamoto Masashi
- Faculty of Agriculture, Kagoshima University
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- Takada Norio
- National Institute of Fruit Tree Science
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- Hirabayashi Toshio
- National Institute of Fruit Tree Science
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- Kubo Tatsuya
- Faculty of Agriculture, Kagoshima University
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- Tominaga Shigeto
- Faculty of Agriculture, Kagoshima University
Bibliographic Information
- Other Title
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- ナシ染色体の蛍光染色
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Abstract
Fluorescent banding patterns of pear chromosomes were determined from samples taken from root tips of open-pollinated seedlings of six cultivars from three species [Pyrus pyrifolia (Burm. F) Nakai (Japanese pear), P. communis L. (European pear), and P. bretschneideri Rehder (Chinese pear)]. Root tips were pretreated in 2 mM 8-hydroxyquinoline at 10°C for 4 h; the chromosome samples were prepared by the enzymatic maceration and air-drying method. All cultivars used in this study had 2n = 34 chromosomes. Chromomycin A3 (CMA) positive (+) bands were observed in telomeric positions of four chromosomes. In some samples, these CMA+bands were observed at satellite positions. 4'-6-diamidino-2-phenylindole (DAPI) negative bands (−) were seen to correspond with CMA+bands. Thirty chromosomes had no CMA+/DAPI−bands. No propidium iodide (PI) bands were observed in any chromosomes. The number and positions of CMA+bands were stable, and there was no difference among the three species.<br>
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 79 (1), 23-26, 2010
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390001205289695744
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- NII Article ID
- 40016940762
- 130004510691
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- NII Book ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- HANDLE
- 10232/18906
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- NDL BIB ID
- 10526977
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed