Ectopic Calcification is Caused by Elevated Levels of Serum Inorganic Phosphate in Mdx Mice
-
- Kikkawa Namiko
- Graduate School of Arts and Sciences, The University of Tokyo
-
- Ohno Tomohisa
- Graduate School of Arts and Sciences, The University of Tokyo
-
- Nagata Yosuke
- Graduate School of Arts and Sciences, The University of Tokyo
-
- Shiozuka Masataka
- Graduate School of Arts and Sciences, The University of Tokyo
-
- Kogure Toshihiro
- Department of Earth and Planetary Science, Graduate School of Science, The University of Tokyo
-
- Matsuda Ryoichi
- Graduate School of Arts and Sciences, The University of Tokyo
Search this article
Abstract
Ectopic calcification occurs in the skeletal muscle of mdx mice, a dystrophin-deficient animal model of Duchenne muscular dystrophy. The purpose of this study was to clarify the mechanism of the calcification. The calcified deposits were identified as hydroxyapatite, a crystallized form of calcium phosphate, and the serum inorganic phosphate (Pi) level in the mdx mice was approximately 1.4 times higher than that in the normal B10 mice, suggesting that Pi plays a critical role in the ectopic calcification. When C2C12 mouse myoblasts were cultured under high-Pi conditions, myogenic differentiation was retarded while the expression of osteogenic markers such as osteocalcin and Runx2 were upregulated. This was followed by the generation of calcium deposition. Moreover, ectopic calcification reduced to an undetectable level in most of the mdx mice fed a Pi-reduced diet. We therefore conclude that the Pi-induced osteogenesis of muscle cells is responsible for ectopic calcification in the skeletal muscle of mdx mice.<br>
Journal
-
- Cell Structure and Function
-
Cell Structure and Function 34 (2), 77-88, 2009
Japan Society for Cell Biology
- Tweet
Details 詳細情報について
-
- CRID
- 1390001204694406272
-
- NII Article ID
- 130004137546
- 40017160766
-
- NII Book ID
- AA0060007X
-
- ISSN
- 13473700
- 03867196
-
- NDL BIB ID
- 10726831
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed