Examination Concerning the Site of Action for the Electrical Barrier of the Respiratory Tract Mucosa Epithelium in Guinea Pigs

• Kohyama Kenya

  Department of Respiratory Medicine, Koshigaya Hospital, Dokkyo Medical University

• Miwa Masato

  Department of Otolaryngology, Koshigaya Hospital, Dokkyo Medical University

• Wakayama Tomoshige

  Department of Respiratory Medicine, Koshigaya Hospital, Dokkyo Medical University

• Kohyama Akemi

  Department of Respiratory Medicine, Koshigaya Hospital, Dokkyo Medical University

• Ikegami Gaku

  Department of Respiratory Medicine, Koshigaya Hospital, Dokkyo Medical University

• Sagara Hironori

  Department of Respiratory Medicine, Koshigaya Hospital, Dokkyo Medical University

• Watanabe Kensuke

  Department of Otolaryngology, Koshigaya Hospital, Dokkyo Medical University

Bibliographic Information

Other Title

• 気道粘膜上皮電気的バリアに対する酸の作用点についての検討
• キドウ ネンマク ジョウヒ デンキテキ バリア ニ タイスル サン ノ サヨウテン ニ ツイテ ノ ケントウ

Abstract

The effect of acid on respiratory epithelium barrier function is still obscure. We attempted to evaluate the electrical barrier function of the trachea in guinea pigs using an Ussing chamber. We exposed the apical membrane side to hydrochloric acid, and then administered DPC (diphenylamine-2-carboxylic acid), and measured the change in the short-circuit current. Similarly, we measured for amiloride, which is a sodium channel blocker, and for zinc chloride, which is a proton channel blocker. The increase in the short-circuit current after acid exposure was partially inhibited by DPC, amiloride, and zinc chloride, but did not show a significant difference in the case of DPC or amiloride. We also found that the decrease in short-circuit current showed a significant difference for zinc chloride. The acid influenced both the transcellular pathway and the paracellular pathway in the epithelium electrical barrier, and it may also reduce epithelial barrier function.

Journal

• Nihon Kikan Shokudoka Gakkai Kaiho

  Nihon Kikan Shokudoka Gakkai Kaiho 61 (6), 493-497, 2010

  The Japan Broncho-esophagological Society

Keywords

• gastro-esophageal reflux disease (GERD)
• proton pump inhibitor
• DPC (diphenylamine-2-carboxylic acid)
• amiloride
• epithelial barrier

Details

• CRID: 1390001205013257856
• NII Article ID: 40017408140
• NII Article ID: 130004470117
• NII Book ID: AN00187474
• DOI: 10.2468/jbes.61.493
• ISSN: 18806848
• ISSN: 00290645
• NDL BIB ID: 10922473
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10922473
• Web Site: https://search.jamas.or.jp/link/ui/2011063790
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed