Label-Free Acetylcholine Image Sensor Based on Charge Transfer Technology for Biological Phenomenon Tracking

• Shoko Takenaga

• Yui Tamai

• Koichi Okumura

Abstract

A 32 ×32 charge-transfer enzyme-type acetylcholine (ACh) image sensor array was produced for label-free tracking of images of ACh distribution and its performance in repeatable measurements without enzyme deactivation was examined. The proposed sensor was based on a charge-transfer-type pH image sensor, which was modified using an enzyme membrane (acetylcholine esterase, AChE) for each pixel. The ACh image sensor detected hydrogen ions generated by the ACh–AChE reaction. A polyion complex membrane composed of poly(L-lysine) and poly(4-styrenesulfonate) was used to immobilize the enzyme on the sensor. The improved uniformity and adhesion of the polyion complex membrane were evaluated in this study. As a result, temporal and spatial fluctuations of the ACh image sensor were successfully minimized using this approach. The sensitivity of the sensor was 4.2 mV/mM, and its detection limit was 20 µM. In five repeated measurements, the repeatability was 8.8%.

Journal

• Japanese journal of applied physics : JJAP

  Japanese journal of applied physics : JJAP 51 (2), 027001-, 2012-02

  Tokyo : The Japan Society of Applied Physics

Keywords

• General Physics and Astronomy
• General Engineering

Details

• CRID: 1520572358945610112
• NII Article ID: 40019171107
• NII Book ID: AA12295836
• ISSN: 00214922
• ISSN: 13474065
• DOI: 10.1143/jjap.51.027001
• NDL BIB ID: 023455322
• Web Site: http://id.ndl.go.jp/bib/023455322
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023455322
• Web Site: https://iopscience.iop.org/article/10.1143/JJAP.51.027001
• Web Site: https://iopscience.iop.org/article/10.1143/JJAP.51.027001/pdf
• Text Lang: en
• NDL Source Classification:
  • ZM35(科学技術--物理学)
• Data Source:
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN