Evaluation of anti-herbivory genes using an Agrobacterium-mediated transient expression system

• Kawazu Kei

  National Institute for Agro-Environmental Sciences

• Wasano Naoya

  National Institute for Agro-Environmental Sciences

• Konno Kotaro

  Plant-microbe Interactions Research Unit, National Institute of Agrobiological Science

• Ohashi Yuko

  Plant-microbe Interactions Research Unit, National Institute of Agrobiological Science

• Mochizuki Atsushi

  National Institute for Agro-Environmental Sciences

• Mitsuhara Ichiro

  Plant-microbe Interactions Research Unit, National Institute of Agrobiological Science

書誌事項

タイトル別名

• Evaluation of anti-herbivory genes using an <i>Agrobacterium</i>-mediated transient expression system

抄録

A high-throughput system for the evaluation of anti-herbivory genes in plant is proposed, in which genes are transiently expressed in a small area of leaf via Agrobacterium-infiltration, and the results can be obtained within 1 week. Transient expression of the cry1Ab gene, a well-known Bt toxin gene, in the leaves of tobacco (Nicotiana tabacum cv. Samsun NN) exhibited a lethal effect on both cotton cutworm (Spodoptera litura) and cabbage armyworm (Mamestra brassicae) larvae. In Arabidopsis leaves, this gene also provided potent inhibition of feeding of diamondback moth (Plutella xylostella) larvae. The expression of another anti-herbivory gene, MLX56, in tobacco and tomato (Solanum lycopersium cv. Micro-Tom) leaves resulted in enhanced mortality of M. brassicae and S. litura larvae. Significantly higher growth inhibition of S. litura larvae and feeding inhibition of P. xylostella larvae were also observed in tobacco and Arabidopsis leaves expressing the MLX56 gene. All results could be obtained within 5–7 days, indicating that this Agrobacterium-mediated transient gene expression system enables a high throughput evaluation of anti-herbivory genes.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 29 (5), 495-499, 2012

  日本植物バイオテクノロジー学会

キーワード

• Anti-herbivory gene
• BT toxin
• Lepidoptera
• MLX56
• transient assay

詳細情報

• CRID: 1390282679305850368
• NII論文ID: 130002500886
• NII論文ID: 40019545352
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.12.0711a
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 024195646
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024195646
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可