Flow Injection Determination of Urinary Protein Using Fluorescence Quenching of Tetraiodofluorescein
Bibliographic Information
- Other Title
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- Original Paper
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Abstract
A flow injection analysis system with spectrofluorimetric detection has been developed for trace protein in urine. The protein assay is based on the ion-association of protein and tetraiodofluorescein (TIF) in the presence of Triton X-100 at pH 3.2. Although the TIF-protein ion associate produces red color compounds, fluorescence quenching caused by bonding with protein was measured at 560 nm (excitation: 313 nm). The quenching is applied to the protein determination. Linearity range was up to 10 mg/L bovine serum albumin and the correlation coefficient was 0.999. The detection limit (S/N = 3) was 0.6 mg/L with RSD 0.134% (n = 4). The FI system is available for rapid screening in diabetic diagnosis.
Journal
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- Journal of Flow Injection Analysis
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Journal of Flow Injection Analysis 28 (2), 120-, 2011
Japanese Association for Flow Injection Analysis
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Details 詳細情報について
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- CRID
- 1390282763026285056
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- NII Article ID
- 130007430562
- 40019903869
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- NII Book ID
- AA10962565
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- ISSN
- 24337374
- 0911775X
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- NDL BIB ID
- 025072297
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed