DEVELOPMENT OF MEASUREMENT TECHNIQUE OF THREE DIMENSIONAL FLOW FIELDS AROUND A MICROBE BY USING A DIFFERENTIAL INTERFERENCE CONTRAST MICROSCOPE AND A SINGLE CAMERA

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  • 微分干渉顕微鏡と単一カメラを用いた微生物周りの３次元流れ場計測法の開発
  • ビブン カンショウ ケンビキョウ ト タンイツ カメラ オ モチイタ ビセイブツ マワリ ノ 3ジゲン ナガレバ ケイソクホウ ノ カイハツ

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Abstract

 To measure velocity distributions around a microbe, the three dimensional Particle Tracking Velocimetry (3D PTV) with a differential interference contrast (DIC) microscope and a single camera has been developed. In this method, the properties of out-of-focus image of a particle are used for estimation of distance from particle to focal plane. In the out-of-focus image of the particle, a ring like image is generated around the center of the particle image and the diameter of the ring like image increases with the distance from the particle to focal plane. Experimental results show that the diameter of the ring like image is proportional to the distance from a particle to the focal plane. The proposed method has been applied to measurements of three dimensional motion of particles around an Euglena in water.

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