酸化モリブデン微粒子の表面増強フォトクロミック現象を利用したα-アミノ酸のラベルフリー定量法の開発

• 山本 麻実

  山口大学大学院理工学研究科

• 安達 健太

  山口大学大学院創成科学研究科 山口大学光・エネルギー研究センター

書誌事項

タイトル別名

• Label-free Colorimetric Sensing of α-Amino Acids Based on Surface-enhanced Photochromic Phenomena of Molybdenum(VI) Oxide Nanoparticles
• サンカ モリブデン ビリュウシ ノ ヒョウメン ゾウキョウ フォトクロミック ゲンショウ オ リヨウ シタ a-アミノサン ノ ラベルフリー テイリョウホウ ノ カイハツ

抄録

Due to the biological importance of α-amino acids and their derivatives, the development of novel colorimetric probes for these molecules has been an active research area in recent years. In this study, surface-enhanced photochromic phenomena by various α-amino acids (L-aspartic acid [Asp], L-leucine [Leu], and L-lysine [Lys]) adsorbed on molybdenum(VI) oxide (MoO₃) nanoparticles in an aqueous system were investigated by means of UV-vis absorption spectrometry. No derivatization of α-amino acids is required, and eventually highly accurate determinations are obtained from the UV-induced MoO₃ photochromic coloration. Under optimal conditions in this system, limit of quantitation (LOQ) values of 3.45 × 10⁻⁶, 2.08 × 10⁻⁶, and 1.53 × 10⁻⁶ mol dm⁻³ for Asp, Leu, and Lys, respectively, were achieved at pH 2. During UV irradiation, the detection process could be visually observed by naked eyes. A simple and unique approach to explore sensitive "label-free" colorimetric sensing toward α-amino acids is proposed.

収録刊行物

• 分析化学

  分析化学 66 (9), 639-646, 2017

  公益社団法人 日本分析化学会

キーワード

• α-amino acid
• molybdenum(VI) oxide
• adsorption
• photochromism
• label-free colorimetric sensing

詳細情報

• CRID: 1390001204662021120
• NII論文ID: 130006135722
• NII書誌ID: AN00222633
• DOI: 10.2116/bunsekikagaku.66.639
• NDL書誌ID: 028544975
• ISSN: 05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028544975
• Web Site: https://www.jstage.jst.go.jp/article/bunsekikagaku/66/9/66_639/_pdf
• 本文言語コード: ja
• データソース種別:
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