Laxative effect of repeated Daiokanzoto is attributable to decrease in aquaporin-3 expression in the colon

• Risako Kon

• Miho Yamamura

• Yukari Matsunaga

• Hiroshi Kimura

• Moe Minami

• Saki Kato

• Nobutomo Ikarashi

• Kiyoshi Sugiyama

Abstract

Daiokanzoto (DKT) exerts its laxative effect via colonic inflammation caused by sennoside A in Daio (rhubarb). Previously, we showed that the laxative effect of sennoside A is related to decreased aquaporin-3 (AQP3) expression in mucosal epithelial cells due to colonic inflammation. We also found that a combination of glycyrrhizin, an ingredient in Kanzo (glycyrrhiza), and sennoside A attenuates the inflammatory response induced by sennoside A and reduces its laxative effect. These findings indicate that DKT may be a long-term treatment for chronic constipation, but there is no evidence supporting this hypothesis. In this study, we analyzed the laxative effect of repeated DKT administration, focusing on AQP3 expression in the colon. After rats were treated for 7 days, decreased AQP3 expression and the onset of diarrhea were observed in the DKT group, but were not seen in the Daio group either. Although the relative abundance of gut microbiota after repeated DKT administration was similar to that after control treatment, Daio reduced Lactobacillaceae , Bifidobacteriaceae , and Bacteroidaceae levels and markedly increased Lachnospiraceae levels. In this study, we show that DKT has a sustained laxative effect, even upon repeated use, probably because it maintains decreased AQP3 expression and gut microbiota homeostasis. This outcome therefore indicates that DKT can be used as a long-term treatment for chronic constipation.

Journal

• Journal of natural medicines / The Japanese Society of Pharmacognosy

  Journal of natural medicines / The Japanese Society of Pharmacognosy 72 (2), 493-502, 2018

  Tokyo : Springer Japan Production Department

Keywords

• Daiokanzoto
• Aquaporin
• Colon
• Bile acid
• Microbiota

Details

• CRID: 1523106605489830016
• NII Article ID: 40021511389
• NII Book ID: AA12114658
• ISSN: 13403443
• ISSN: 18610293
• DOI: 10.1007/s11418-018-1174-1
• NDL BIB ID: 028918219
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028918219
• Web Site: http://link.springer.com/article/10.1007/s11418-018-1174-1/fulltext.html
• Web Site: http://link.springer.com/content/pdf/10.1007/s11418-018-1174-1.pdf
• Web Site: https://search.jamas.or.jp/link/ui/2019042942
• Text Lang: en
• NDL Source Classification:
  • ZS51(科学技術--薬学)
• Data Source:
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN