Effect of Heat Stress on the Quality of Cumulus-Oocyte Complex Obtained by Ovum Pick-Up in Japanese Black Cows

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  • EGASHIRA Junki
    The United Graduate School of Agricultural Sciences, Kagoshima University, Japan Saga Prefectural Livestock Experimental Station, Japan
  • TATEMOTO Hideki
    The United Graduate School of Agricultural Sciences, Kagoshima University, Japan Faculty of Agriculture, University of Ryukyus, Japan
  • WADA Yasuhiko
    The United Graduate School of Agricultural Sciences, Kagoshima University, Japan Faculty of Agriculture, Saga University, Japan
  • YAMANAKA Ken-ichi
    The United Graduate School of Agricultural Sciences, Kagoshima University, Japan Faculty of Agriculture, Saga University, Japan

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Other Title
  • 暑熱ストレスが経膣採卵により採取されたウシ卵丘－卵母細胞複合体の品質に及ぼす影響
  • ショネツ ストレス ガ ケイチツサイラン ニ ヨリ サイシュ サレタ ウシラン オカ-ランボ サイボウ フクゴウタイ ノ ヒンシツ ニ オヨボス エイキョウ

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Abstract

<p>In the present study, we addressed the low conception rate during hot season in cows and conducted to clarify the seasonal difference in the quality of cumulus-oocyte complex （COCs）. The COCs were collected from Japanese black cows by the ovum pick-up （OPU） method during the hot （August to September, 2016） and cool （February to March, 2017） seasons. The OPU was performed 15 and 18 times in 5 and 3 cows during hot and cool season, respectively. The rectal temperature and respiration rate, and follicular number of cows at the time of OPU were measured. After OPU, the number and morphological grade of the collected COCs were evaluated. Moreover, the number of apoptotic cells in cumulus cells and mitochondrial distribution in oocytes were also analyzed. The number and morphological grade of COCs did not differ between the hot and cool seasons, although the rectal temperature and respiration rate of cows was significantly increased during hot season （P < 0.01）. On the other hand, number of apoptotic cells in cumulus cells was significantly higher in COCs obtained during hot season than that of cool season（P < 0.01）. Likewise, the rate of normal mitochondrial distribution observed in oocytes was significantly decreased in hot season compared with that of cool season （P < 0.05）. These findings indicate that heat stress would decrease the quality of COCs in Japanese Black cows.</p>

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