Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan

• Madoka Kuramitsu

• Kazu Okuma

• Makoto Nakashima

• Tomoo Sato

• Daisuke Sasaki

• Hiroo Hasegawa

• Kazumi Umeki

• Ryuji Kubota

• Keiko Sasada

• Rieko Sobata

• Chieko Matsumoto

• Noriaki Kaneko

• Kenta Tezuka

• Sahoko Matsuoka

• Atae Utsunomiya

• Ki-Ryang Koh

• Masao Ogata

• Kenji Ishitsuka

• Mai Taki

• Kisato Nosaka

• Kaoru Uchimaru

• Masako Iwanaga

• Yasuko Sagara

• Yoshihisa Yamano

• Akihiko Okayama

• Kiyonori Miura

• Masahiro Satake

• Shigeru Saito

• Toshiki Watanabe

• Isao Hamaguchi

Abstract

<jats:label /> Quantitative PCR (qPCR) of human T‐cell leukemia virus type 1 (HTLV‐1) provirus is used for HTLV‐1 testing and for assessment of risk of HTLV‐1‐related diseases. In this study, a reference material was developed for standardizing HTLV‐1 qPCR. Freeze‐dried TL‐Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2‐fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.

Journal

• Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research

  Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research 62 (10), 673-676, 2018-10

  Richmond : Blackwell Publishing Asia Pty

Keywords

• human T-cell leukemia virus type 1
• proviral load
• quantitative PCR
• standard

Details

• CRID: 1522543655090228096
• NII Article ID: 40021702629
• NII Book ID: AA00738350
• ISSN: 03855600
• ISSN: 13480421
• DOI: 10.1111/1348-0421.12644
• NDL BIB ID: 029304758
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029304758
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1348-0421.12644
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/1348-0421.12644
• Text Lang: en
• NDL Source Classification:
  • ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
• Data Source:
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN