Generation of a purely clonal defective interfering influenza virus

• Yutaro Yamagata

• Yukiko Muramoto

• Sho Miyamoto

• Keiko Shindo

• Masahiro Nakano

• Takeshi Noda

Abstract

Defective interfering (DI) influenza viruses carry a large deletion in a gene segment that interferes with the replication of infectious virus; thus, such viruses have potential for antiviral therapy. However, because DI viruses cannot replicate autonomously without the aid of an infectious helper virus, clonal DI virus stocks that are not contaminated with helper virus have not yet been generated. To overcome this problem, we used reverse genetics to generate a clonal DI virus with a PB2 DI gene, amplified the clonal DI virus using a cell line stably expressing the PB2 protein, and confirmed its ability to interfere with infectious virus replication in vitro. Thus, our approach is suitable for obtaining purely clonal DI viruses, will contribute to the understanding of DI virus interference mechanisms and can be used to develop DI virus‐based antivirals.

Journal

• Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research

  Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research 63 (5), 164-171, 2019-05

  Richmond : Blackwell Publishing Asia Pty

Keywords

• clonal defective interfering virus
• influenza virus

Details

• CRID: 1523106604879309696
• NII Article ID: 40021927501
• NII Book ID: AA00738350
• ISSN: 03855600
• ISSN: 13480421
• DOI: 10.1111/1348-0421.12681
• NDL BIB ID: 029762108
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029762108
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1348-0421.12681
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/1348-0421.12681
• Web Site: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1348-0421.12681
• Text Lang: en
• NDL Source Classification:
  • ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
• Data Source:
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN