Bordet-Gengou agar medium supplemented with albumin-containing biologics for cultivation of bordetellae

• Yukihiro Hiramatsu

• Mayuko Osada-Oka

• Yasuhiko Horiguchi

Abstract

Bordetella pertussis , B. parapertussis , and B. bronchiseptica cause respiratory infections in mammals, including humans, and are generally cultivated on Bordet‐Gengou (BG) agar plates in laboratories. The medium requires animal blood as a supplement for better bacterial growth. However, using blood is problematic, as its constant supply is occasionally difficult because of the limited shelf‐life. This study proposes modified BG agar plates supplemented with bovine serum albumin and fetal bovine serum as a simple and convenient medium that confers sufficient growth of bordetellae.

Journal

• Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research

  Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research 63 (12), 513-516, 2019-12

  Richmond : Blackwell Publishing Asia Pty

Keywords

• bordetellae
• Bordet-Gengou agar plate
• bovine serum albumin
• fetal bovine serum

Details

• CRID: 1520010380338941312
• NII Article ID: 40022103554
• NII Book ID: AA00738350
• ISSN: 03855600
• ISSN: 13480421
• DOI: 10.1111/1348-0421.12742
• NDL BIB ID: 030147508
• Web Site: http://id.ndl.go.jp/bib/030147508
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030147508
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1348-0421.12742
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/1348-0421.12742
• Web Site: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1348-0421.12742
• Web Site: https://search.jamas.or.jp/link/ui/2020287437
• Text Lang: en
• NDL Source Classification:
  • ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
• Data Source:
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN