Identification of the Epitope Recognized by the Anti-Red Sea Bream Iridovirus (RSIV) Monoclonal Antibody M10 Using a Phage Display RSIV Peptide Library

DOI Web Site Web Site 参考文献25件
  • Takano Tomokazu
    Nansei Main Station, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency
  • Matsuyama Tomomasa
    Nansei Main Station, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency
  • Kawato Yasuhiko
    Nansei Main Station, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency
  • Sakai Takamitsu
    Tamaki Laboratory, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency
  • Kurita Jun
    Tamaki Laboratory, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency
  • Matsuura Yuta
    Nansei Main Station, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency
  • Terashima Sachiko
    Nansei Main Station, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency
  • Nakajima Kazuhiro
    National Research Institute of Fisheries and Environment of Inland Sea, Japan Fisheries Research and Education Agency
  • Nakayasu Chihaya
    Nansei Main Station, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency

この論文をさがす

抄録

<p>Red sea bream iridoviral disease (RSIVD) spreads readily throughout the marine environment because of its wide variety of host species. Use of a rapid diagnostic method, such as the immunofluorescence antibody test, helps to control the disease, so the anti-RSIV monoclonal antibody M10 (mAb M10) has been developed in Japan. In the present study, we carried out epitope mapping using the phage display method to identify the antigen of mAb M10. A phage display RSIV peptide library was constructed to cover the entire genome of RSIV, then phage clones recognized by the antibody were selected by biopanning. The selected clones harbored partial fragments of the laminin-type epidermal growth factor-like domain (LEGFD) gene. N-terminal and C-terminal deletion peptides were then prepared from the amino acid sequence deduced from the smallest fragment to precisely determine the epitope. Finally, seven amino acids, EYDCPEY, located in the extracellular domain of the LEGFD protein were determined to be the epitope. Identical residues of the epitope were also identified from the LEGFD protein in other megalocytiviruses including the infectious spleen and kidney necrosis virus and turbot reddish body iridovirus. mAb M10 is considered to be widely available for the diagnostics of megalocytivirus infections.</p>

収録刊行物

  • 魚病研究

    魚病研究 54 (4), 83-92, 2020-01-15

    日本魚病学会

参考文献 (25)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ