Feeding ecology of a mysid species, Neomysis awatschensis in the Lake Kasumigaura : combining approach with microscopy, stable isotope analysis and DNA metabarcoding

• Nakamura Yasuhide

  Department of Botany, National Museum of Nature and Science

• Tuji Akihiro

  Department of Botany, National Museum of Nature and Science

• Makino Wataru

  Graduate School of Life Sciences, Tohoku University

• Matsuzaki Shin-ichiro S.

  Center for Environmental Biology & Ecosystem Studies, National Institution for Environmental Studies

• Nagata Nobuaki

  Division of Collections Conservation, National Museum of Nature and Science

• Nakagawa Megumi

  Center for Environmental Biology & Ecosystem Studies, National Institution for Environmental Studies

• Takamura Noriko

  Center for Environmental Biology & Ecosystem Studies, National Institution for Environmental Studies

書誌事項

タイトル別名

• Feeding ecology of a mysid species, <i>Neomysis awatschensis</i> in the Lake Kasumigaura: combining approach with microscopy, stable isotope analysis and DNA metabarcoding

抄録

Neomysis awatschensis is an important prey item for various fishes in Lake Kasumigaura, Japan. There is, however, a contradiction concerning the major food sources of this mysid species: whether “bottom mud”, “particulate organic matter (POM) including phytoplankton”, or “mesozooplankton” is the main diet, and this uncertainty may be due to differences in the methodology used to determine the prey items in previous studies. This study examined the main food sources of N. awatschensis by combining three methods to eliminate methodological biases: DNA metabarcoding, microscopy and stable isotope analysis. Planktonic diatoms and green algae sequences were the main taxa detected by DNA metabarcoding and microscopy on the fecal pellets. The δ¹⁵N values of the mysids were similar to those of phytoplankton feeders rather than carnivorous planktonic crustaceans. These results suggest that diatoms and green algae were the major food sources for N. awatschensis in Lake Kasumigaura during the investigation period, and that its trophic level is as low as that of herbivores. However, the partial contribution of other “POM” (e.g., benthic diatoms) is also implied, considering the dispersion in δ¹³C values. Mesozooplankton prey, such as copepods, were detected both using DNA metabarcoding and microscopy, but they could not be a major food source due to the low nitrogen stable isotopic signature of the mysids. The opportunistic feeding habits of N. awatschensis possibly explain the consistent dominance of this species in the lake, where the ecosystem structure was substantially changed because of successive desalination.

収録刊行物

• Plankton and Benthos Research

  Plankton and Benthos Research 15 (1), 44-54, 2020-02-28

  日本プランクトン学会、日本ベントス学会

キーワード

• desalination
• fecal pellets
• Mysida
• Mysidacea
• zooplankton

詳細情報

• CRID: 1390565134839352448
• NII論文ID: 130007815677
• NII書誌ID: AA12130745
• DOI: 10.3800/pbr.15.44
• ISSN: 1882627X
• ISSN: 18808247
• NDL書誌ID: 030281578
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030281578
• Web Site: https://www.jstage.jst.go.jp/article/pbr/15/1/15_P150107/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可