The effect of temperature on environmental DNA degradation of Japanese eel

• Akihide Kasai

• Shingo Takada

• Aya Yamazaki

• Reiji Masuda

• Hiroki Yamanaka

抄録

The environmental DNA (eDNA) technique is a convenient and powerful tool to detect rare species. Knowledge of the degradation rate of eDNA in water is important for understanding how degradation influences the presence and/or estimate biomass of aquatic animals. We developed a new set of species-specific primers and probe to detect eDNA of Japanese eel Anguilla japonica , which is a commercially important and endangered species, and then conducted a laboratory experiment to quantify the temperature-dependent degradation of emitted eDNA. Eels were held in tanks at five different temperature levels from 10 to 30 °C and water from each tank was sampled and kept in bottles at each temperature over 6 days. The concentration of eDNA was measured every day and the results showed that temperature ( T ) had a significant and positive effect on the degradation rate ( k ) as k  = 0.02 T  + 0.18. Improved understanding of the effect of temperature on degradation rates would help data interpretations and adjustments would increase the reliability of eDNA analysis in future studies.

収録刊行物

• Fisheries science / Japanese Society of Fisheries Science

  Fisheries science / Japanese Society of Fisheries Science 86 (3), 465-471, 2020-05

  [Tokyo] : [Springer Japan]

キーワード

• Environmental DNA
• Degradation rate
• Temperature
• Primer
• Japanese eel

詳細情報

• CRID: 1523669555618769920
• NII論文ID: 40022230778
• NII書誌ID: AA10993718
• ISSN: 09199268
• ISSN: 14442906
• DOI: 10.1007/s12562-020-01409-1
• NDL書誌ID: 030410426
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030410426
• Web Site: https://link.springer.com/content/pdf/10.1007/s12562-020-01409-1.pdf
• Web Site: https://link.springer.com/article/10.1007/s12562-020-01409-1/fulltext.html
• 本文言語コード: en
• NDL 雑誌分類:
  • ZR26(科学技術--農林水産--水産)
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  • NDL
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