Linker DNA and histone contributions in nucleosome binding by p53

• Masahiro Nishimura

• Yasuhiro Arimura

• Kayo Nozawa

• Hitoshi Kurumizaka

抄録

The tumour suppressor protein p53 regulates various genes involved in cell-cycle arrest, apoptosis and DNA repair in response to cellular stress, and apparently functions as a pioneer transcription factor. The pioneer transcription factors can bind nucleosomal DNA, where many transcription factors are largely restricted. However, the mechanisms by which p53 recognizes the nucleosomal DNA are poorly understood. In the present study, we found that p53 requires linker DNAs for the efficient formation of p53-nucleosome complexes. p53 forms an additional specific complex with the nucleosome, when the p53 binding sequence is located around the entry/exit region of the nucleosomal DNA. We also showed that p53 directly binds to the histone H3-H4 complex via its N-terminal 1–93 amino acid region. These results shed light on the mechanism of nucleosome recognition by p53.

収録刊行物

• The journal of biochemistry

  The journal of biochemistry 168 (6), 669-675, 2020-12

  Tokyo : Japanese Biochemical Society

キーワード

• chromatin
• histone
• nucleosome
• p53
• pioneer transcription factor

詳細情報

• CRID: 1520854806028556800
• NII論文ID: 40022454706
• NII書誌ID: AA00694073
• ISSN: 0021924X
• ISSN: 17562651
• DOI: 10.1093/jb/mvaa081
• NDL書誌ID: 031224315
• Web Site: http://id.ndl.go.jp/bib/031224315
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I031224315
• Web Site: http://academic.oup.com/jb/advance-article-pdf/doi/10.1093/jb/mvaa081/33732610/mvaa081.pdf
• Web Site: http://academic.oup.com/jb/article-pdf/168/6/669/35149042/mvaa081.pdf
• Web Site: https://search.jamas.or.jp/link/ui/2022000039
• 本文言語コード: en
• NDL 雑誌分類:
  • ZR2(科学技術--生物学--生化学)
• データソース種別:
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