Correlation between seed longevity and RNA integrity in the embryos of rice seeds

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  • Saighani Kalimullah
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
  • Kondo Daiki
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
  • Sano Naoto
    Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay
  • Murata Kazumasa
    Toyama Prefectural Agricultural, Forestry & Fisheries Research Center
  • Yamada Tetsuya
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
  • Kanekatsu Motoki
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology

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<p>The mature embryos of rice seeds contain translatable mRNAs required for the initial phase of germination. To clarify the relationship between seed longevity and RNA integrity in embryos, germinability and stability of embryonic RNAs were analyzed using the seeds of japonica rice cultivars subjected to controlled deterioration treatment (CDT) or long periods of storage. Degradation of RNA from embryos of a japonica rice cultivar “Nipponbare” was induced by CDT before the decline of the germination rate and we observed a positive relationship between seed germinability and integrity of embryonic RNAs. Moreover, this relationship was confirmed in the experiments using aged seeds from the “Nipponbare”, “Sasanishiki” and “Koshihikari” rice cultivars. In addition, the RNA integrity number (RIN) values, calculated using electrophoresis data and Agilent Bioanalyzer software, had a positive correlation with germinability (R2=0.75). Therefore, the stability of embryonic RNAs required for germination is involved in maintaining seed longevity over time and RIN values can serve as a quantitative indicator to evaluate germinability in rice.</p>

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