書誌事項
- タイトル別名
-
- Estimation of genome composition of F3 hybrids among carp, crucian carp, and goldfish using PCR methods.
- PCRを用いたコイとフナ類およびキンギョの雑種F₃が有するゲノムの推定
- PCR オ モチイタ コイ ト フナルイ オヨビ キンギョ ノ ザッシュ F ₃ ガ ユウスル ゲノム ノ スイテイ
この論文をさがす
抄録
Carp（Cyprinus carpio）, Carassius fish, and goldfish（Carassius auratus）are popular cyprinid species bred for use in aquaculture as a food source, in aquaria, and biological research in many countries. Hybrids derived from a cross between carp and Carassius fish containing the genome of crucian carp and goldfish have been artificially produced for use as food and aquarium fish. We obtained a third generation of diploid and triploid hybrids（F3）derived from crosses between the F2 hybrid of family 1［F1 hybrid（C. carpio ♀ × Carassius cuvieri ♂）♀ × C. carpio ♂］♀ and C. auratus ♂ and cross between the F2 hybrid of family 2［F1 hybrid（C. carpio ♀ × C. cuvieri ♂）♀ × C. cuvieri ♂ ］ ♀ and C. auratus ♂. However, it is difficult to estimate the genome composition of the F3 hybrids by morphological measurement. We previously developed three methods of species distinction between carp and Carassius fish：（1）analysis by PCR amplification using carp- and crucian carp-specific primers targeting the IGF-1 gene region,（2）PCR-RFLP analysis of the TGF-beta gene region, and（3）PCR-RFLP analysis of the mitochondrial 16S ribosomal RNA gene region. In this study, we estimated the genome composition of eight F3 hybrids by these methods. We estimated that five F3 hybrids contained the genomes of C. carpio, C. cuvieri, and C. auratus. Thus, the five F3 hybrids are considered to be allotriploids and include the genomes of three fish species. We estimated that two F3 hybrids contained the genomes of C. carpio and C. auratus. We also estimated that one F3 hybrid contained the genomes of C. cuvieri and C. auratus . Thus, these three F3 hybrid types are considered to be diploid hybrids. Moreover, all the eight F3 hybrids were estimated to contain the mitochondrial DNA derived from C. carpio as the maternal species.
収録刊行物
-
- DNA鑑定
-
DNA鑑定 12 27-40, 2021-07-21
横浜 : DNA鑑定学会