Structural studies on glutathione synthetase from Escherichia coli B 大腸菌由来グルタチオン合成酵素の構造研究
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著者
書誌事項
- タイトル
-
Structural studies on glutathione synthetase from Escherichia coli B
- タイトル別名
-
大腸菌由来グルタチオン合成酵素の構造研究
- 著者名
-
山口, 宏
- 著者別名
-
ヤマグチ, ヒロシ
- 学位授与大学
-
大阪大学
- 取得学位
-
理学博士
- 学位授与番号
-
甲第4255号
- 学位授与年月日
-
1991-03-26
注記・抄録
博士論文
目次
- Table of Contents / p5 (0004.jp2)
- Chapter-1. / p1 (0006.jp2)
- General Introduction / p1 (0006.jp2)
- 1.1. Glutathione Synthetase from Escherichia coli B / p1 (0006.jp2)
- Chapter-2. / p6 (0009.jp2)
- Three-dimensional Structure Determination of Glutathione Synthetase from Escherichia coli B / p6 (0009.jp2)
- 2.1. Introduction / p6 (0009.jp2)
- 2.2. Enzyme Preparation / p7 (0009.jp2)
- 2.3. Crystallization and Determination of Crystallographic Data / p8 (0010.jp2)
- 2.4. Preparation of Heavy-atom Derivatives / p13 (0012.jp2)
- 2.5. Data collection on a Four-circle Diffractometer / p17 (0014.jp2)
- 2.6. High Resolution Data Collection of Native Crystals / p20 (0016.jp2)
- 2.7. Initial Determination of Heavy-atom Binding Sites / p24 (0018.jp2)
- 2.8. Refinement of Heavy-atom Parameters and Determination of Absolute Configuration / p27 (0019.jp2)
- 2.9. Interpretation of Native Fourier Map and Model Construction / p32 (0022.jp2)
- 2.10. Structure Refinement / p33 (0022.jp2)
- Chapter-3. / p42 (0027.jp2)
- Structure of Glutathione Synthetase / p42 (0027.jp2)
- 3.1. Introduction / p42 (0027.jp2)
- 3.2. Subunit Structure / p42 (0027.jp2)
- 3.3. Quaternary Structure / p47 (0029.jp2)
- Chapter-4. / p56 (0034.jp2)
- Structure Determination of Glutathione Synthetase Crystallized fromTris Buffer System / p56 (0034.jp2)
- 4.1. Introduction / p56 (0034.jp2)
- 4.2. Data Collection / p57 (0034.jp2)
- 4.3. Structure Refinement / p59 (0035.jp2)
- 4.4. Comparison between the Structures from both the Solutions of Phosphate Buffer and Tris Buffer / p62 (0037.jp2)
- Chapter-5. / p67 (0039.jp2)
- Substrate Binding Sites / p67 (0039.jp2)
- 5.1. Introduction / p67 (0039.jp2)
- 5.2. Preparation of Complex Crystals / p67 (0039.jp2)
- 5.3. Data Collection / p68 (0040.jp2)
- 5.4. Determination of Substrate Binding Sites / p71 (0041.jp2)
- 5.5. ATP Binding Site / p79 (0045.jp2)
- 5.6. γ-L-Glutamyl-L-Aminobutylate Binding Site / p81 (0046.jp2)
- Chapter-6. / p84 (0048.jp2)
- A Role of the Flexible Loop / p84 (0048.jp2)
- 6.1. Introduction / p84 (0048.jp2)
- 6.2. Arginylendopeptidase Cleavage / p85 (0048.jp2)
- 6.3. Mutational Analysis / p91 (0051.jp2)
- 6.4. Discussion in the role of the flexible loop / p105 (0058.jp2)
- Chapter-7. / p107 (0059.jp2)
- Conclusion and Summary / p107 (0059.jp2)
- Appendix / p111 (0061.jp2)
- ABBREVIATIONS / p113 (0062.jp2)
- REFERENCES / p116 (0064.jp2)
- LIST OF PUBLICATIONS / p120 (0066.jp2)
