Chemical and physical properties of lipids and proteins from Amaranthus cruentus seed アマランタス・クルエンタス種子の脂質及びタンパク質の化学及び物理的特性に関する研究
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Bibliographic Information
- Title
-
Chemical and physical properties of lipids and proteins from Amaranthus cruentus seed
- Other Title
-
アマランタス・クルエンタス種子の脂質及びタンパク質の化学及び物理的特性に関する研究
- Author
-
Jorge Soriano, Santos
- Author(Another name)
-
ホルヘ ソリアノ, サントス
- University
-
東北大学
- Types of degree
-
農学博士
- Grant ID
-
甲第4468号
- Degree year
-
1991-03-28
Note and Description
博士論文
Table of Contents
- CONTENTS / p1 (0005.jp2)
- INTRODUCTION / p1 (0011.jp2)
- Chapter I.Characteristics of Amaranthus cruentus seed oil and its oxidative stability / p5 (0015.jp2)
- Materials and methods / p5 (0015.jp2)
- Materials / p5 (0015.jp2)
- Lipid isolation / p5 (0015.jp2)
- Chemical analyses / p7 (0017.jp2)
- Separation of neutral and polar lipid classes / p7 (0017.jp2)
- Fractionation of each lipid class / p7 (0017.jp2)
- Fatty acid composition of lipid classes / p9 (0019.jp2)
- Tocopherol composition / p10 (0020.jp2)
- Oxidative stability / p10 (0020.jp2)
- Results and discussion / p12 (0022.jp2)
- Chemical composition of oil from Amaranthus cruentus seed / p12 (0022.jp2)
- Lipid class composition of amaranth oil / p12 (0022.jp2)
- Fatty acid composition of lipid class from Amaranthus cruentus Seed Oil / p19 (0029.jp2)
- Tocopherol composition of oil amaranth seed / p21 (0031.jp2)
- Stability to oxidation of amaranth seed oil / p24 (0034.jp2)
- Summary / p26 (0036.jp2)
- Chapter II.Extraction and solubility of proteins from Amaranthus cruentus Seed / p27 (0037.jp2)
- Materials and methods / p28 (0038.jp2)
- Materials / p28 (0038.jp2)
- Sample preparation / p29 (0039.jp2)
- Protein extraction using different salts / p29 (0039.jp2)
- Protein fractionation / p30 (0040.jp2)
- Nitrogen solubility test / p30 (0040.jp2)
- pH dependent precipitation of albumin and globulin fractions / p33 (0043.jp2)
- Results and discussion / p35 (0045.jp2)
- Efficiency of protein extraction / p35 (0045.jp2)
- Selection of protein solubilizer / p37 (0047.jp2)
- Differences in solubilization of nitrogen between sodium chloride and sodium sulfate / p39 (0049.jp2)
- Yield of protein fractionation / p40 (0050.jp2)
- Nitrogen solubility test / p46 (0056.jp2)
- Precipitation of albumin and globulin as a function of pH / p48 (0058.jp2)
- Summary / p52 (0062.jp2)
- Chapter III.Amino-acid profile of Amaranthus cruentus seed protein fractions / p53 (0063.jp2)
- Materials and methods / p54 (0064.jp2)
- Materials / p54 (0064.jp2)
- Heat treatment (popping) / p54 (0064.jp2)
- Determination of amino-acids / p54 (0064.jp2)
- Racemization of amino-acids / p55 (0065.jp2)
- Results and discussion / p56 (0066.jp2)
- Profile of amino-acids / p56 (0066.jp2)
- Racemization of amino-acids / p59 (0069.jp2)
- Summmary / p62 (0072.jp2)
- Chapter IV.Characterization of albumin and globulin from Amaranthus cruentus Seed / p63 (0073.jp2)
- Materials and methods / p63 (0073.jp2)
- Materials / p63 (0073.jp2)
- Purification of albumin and globulin / p63 (0073.jp2)
- Determination of molecular weight of albumin and globulin / p64 (0074.jp2)
- Polyacrylamide gel electrophoresis (PAGE) / p66 (0076.jp2)
- SDS-PAGE electrophoresis and molecular weight determination / p67 (0077.jp2)
- Determination of trypsin inhibitor / p67 (0077.jp2)
- Glycoproteins analysis / p68 (0078.jp2)
- Results and discussion / p69 (0079.jp2)
- Characterization of albumin and globulin / p69 (0079.jp2)
- Molecular weight of native albumin and globulin from amaranth seed / p79 (0089.jp2)
- Electrophoresis pattern and molecular weight determination of polypeptides from albumin and globulin fractions / p82 (0092.jp2)
- Composed polypeptides from crude albumin and globulin fractions / p91 (0101.jp2)
- Trypsin inhibitor and glycoprotein analysis / p91 (0101.jp2)
- Summary / p97 (0107.jp2)
- Conclusions / p99 (0109.jp2)
- References / p104 (0114.jp2)