Structure and function of the gene coding adenylate cyclase in Neurospora crassa アカパンカビアデニル酸シクラーゼの遺伝子構造と機能
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著者
書誌事項
- タイトル
-
Structure and function of the gene coding adenylate cyclase in Neurospora crassa
- タイトル別名
-
アカパンカビアデニル酸シクラーゼの遺伝子構造と機能
- 著者名
-
是枝, 晋
- 著者別名
-
コレエダ, シン
- 学位授与大学
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東京大学
- 取得学位
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理学博士
- 学位授与番号
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甲第8935号
- 学位授与年月日
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1991-03-29
注記・抄録
博士論文
目次
- TABLE OF CONTENTS / p1 (0003.jp2)
- TABLE OF CONTENTS / p1 (0003.jp2)
- LIST OF TABLES / p4 (0006.jp2)
- LIST OF FIGURES / p5 (0007.jp2)
- ACKNOWLEDGEMENT / p7 (0009.jp2)
- ABBREVIATIONS / p8 (0010.jp2)
- SUMMARY / p1 (0011.jp2)
- INTRODUCTION / p3 (0013.jp2)
- (1)Cyclic AMP as a intracellular regulator / p3 (0013.jp2)
- (2)Physiological roles of cAMP and adenylate cyclase of N.crassa / p3 (0013.jp2)
- (3)Adenylate cyclase of other organisms / p5 (0015.jp2)
- (4)Recent advances in molecular genetical technics for N.crassa / p7 (0017.jp2)
- (5)The aim of this study / p7 (0017.jp2)
- MATERIALS AND METHODS / p9 (0019.jp2)
- (1)Organisms / p9 (0019.jp2)
- (2)Vectors,λ clones and the cosmid library / p9 (0019.jp2)
- (3)Media and growth conditions of N.crassa / p12 (0022.jp2)
- (4)Isolation of DNA from mycelia of N.crassa / p13 (0023.jp2)
- (5)Isolation of poly(A)⁺RNA from N.crassa / p14 (0024.jp2)
- (6)Isolation of plasmid DNA from E.coli and λ phage DNA / p15 (0025.jp2)
- (7)Enzymes for molecular biological studies / p15 (0025.jp2)
- (8)Recovery of DNA from agarose gels / p16 (0026.jp2)
- (9)Hybridization analyses / p16 (0026.jp2)
- (10)Base sequence analysis of DNA / p17 (0027.jp2)
- (11)Synthesis of oligonucleotide / p17 (0027.jp2)
- (12)Primer extension / p17 (0027.jp2)
- (13)RNase mapping / p19 (0029.jp2)
- (14)Synthesis and amplification of cDNA / p19 (0029.jp2)
- (15)Transformation of N.crassa / p20 (0030.jp2)
- (16)Mapping of nac locus by using restriction fragment length polymorphisms / p20 (0030.jp2)
- (17)Disruption of nac gene by using the RIP effect / p21 (0031.jp2)
- (18)Adenylate cyclase assay / p21 (0031.jp2)
- RESULTS / p22 (0032.jp2)
- PART I Structure of an adenylate cyclase gene(nac)from N.crassa / p22 (0032.jp2)
- (1)Isolation of cosmid clones that carries a sequence homologous with the yeast CYR1 gene / p22 (0032.jp2)
- (2)The nucleotide sequence determination of the nac gene / p25 (0035.jp2)
- (3)The transcriptional unit of nac gene and 5'flanking sequence of its coding region / p30 (0040.jp2)
- (4)Identification of boundaries between exons and introns / p35 (0045.jp2)
- (5)Structure of the nac gene product / p40 (0050.jp2)
- (6)Homology of the nac gene product with adenylate cyclases from other organisms / p47 (0057.jp2)
- (7)The nac gene product is an adenylate cyclase / p52 (0062.jp2)
- (8)Mapping of nac locus by using RFLP / p57 (0067.jp2)
- PART II The role of nac gene in morphogenesis during vegetative growth of N.crassa / p59 (0069.jp2)
- (1)The suppression of aberrant morphology of a cr-1 mutant / p59 (0069.jp2)
- (2)The restoration of adenylate cyclase activity in the cr-1 strains transformed with Cos4 / p62 (0072.jp2)
- (3)Disruption of nac gene / p62 (0072.jp2)
- (4)The adenylate cyclase activity is not detectable in the nac mutant strains / p73 (0083.jp2)
- DISCUSSION / p76 (0086.jp2)
- (1)The nac gene has three introns. / p76 (0086.jp2)
- (2)Three types of adenylate cyclases found in eukaryote and prokaryote / p77 (0087.jp2)
- (3)A glutamine-rich region in N-terminal segment / p78 (0088.jp2)
- (4)The nac gene is non-essential for growth of N.crassa mycelia. / p78 (0088.jp2)
- (5)The nac disruptant has phenotype indistinguishable to cr-1 mutant. / p79 (0089.jp2)
- LITERATURE CITED / p81 (0091.jp2)