Molecular biological studies on structure and function of vacuolar membrane H[+] translocating ATPase in Saccharomyces cerevisiae
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Bibliographic Information
- Title
-
Molecular biological studies on structure and function of vacuolar membrane H[+] translocating ATPase in Saccharomyces cerevisiae
- Author
-
梅基, 直行
- Author(Another name)
-
ウメモト, ナオユキ
- University
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東京大学
- Types of degree
-
薬学博士
- Grant ID
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甲第9183号
- Degree year
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1991-03-29
Note and Description
博士論文
Table of Contents
- 論文の内容の要旨 / (0001.jp2)
- TABLE OF CONTENTS / p6 (0011.jp2)
- ACKNOWLEDGEMENTS / p3 (0008.jp2)
- SUMMARY / p4 (0009.jp2)
- TABLE OF CONTENTS / p6 (0011.jp2)
- ABBREVIATIONS / p10 (0015.jp2)
- CHAPTER1:Introduction / p12 (0017.jp2)
- Vacuole / p12 (0017.jp2)
- Yeast vacuole / p13 (0018.jp2)
- Vacuolar membrane H⁺-ATPase in Saccharomyces cerevisiae / p15 (0020.jp2)
- V(acular)-type ATPase / p15 (0020.jp2)
- CHAPTER2:Experimental Procedures / p20 (0025.jp2)
- 2.1 Materials / p20 (0025.jp2)
- 2.2 Strains / p20 (0025.jp2)
- 2.3 Plasmids / p20 (0025.jp2)
- 2.4 Culture condition and yeast genetics / p20 (0025.jp2)
- 2.5 Preparation of vacuolar membrane vesicles / p22 (0027.jp2)
- 2.6 Radioactive DCCD labeling / p23 (0028.jp2)
- 2.7 Isolation of subunit c of vacuolar membrane H⁺-ATPase and peptide sequencing / p23 (0028.jp2)
- 2.8 Recombinant DNA technique / p24 (0029.jp2)
- 2.9 Cloning VMA3 gene / p25 (0030.jp2)
- 2.10 Cloning CLS genes / p26 (0031.jp2)
- 2.11 Enzyme assays / p27 (0032.jp2)
- 2.12 Gel electrophoresis and immunoblotting / p28 (0033.jp2)
- 2.13 Immunofluorescence microscopy / p28 (0033.jp2)
- 2.14 Quinacrine staining / p29 (0034.jp2)
- 2.15 Fractionation of cell content / p29 (0034.jp2)
- 2.16 Extraction of proteolipid from total cell / p29 (0034.jp2)
- 2.17 Protein determination / p30 (0035.jp2)
- CHAPTER3:Cloning and Sequencing of a Gene (VMA3) for Subunit c of Vacuolar Membrane H⁺-ATPase / p37 (0042.jp2)
- 3.1 Introduction / p37 (0042.jp2)
- 3.2 Results / p38 (0043.jp2)
- 3.3 Discussion / p39 (0044.jp2)
- CHAPTER4:Construction and Characterization of Cells Deficient in VMA3 Gene / p53 (0058.jp2)
- 4.1 Introduction / p53 (0058.jp2)
- 4.2 Results / p53 (0058.jp2)
- 4.3 Discussion / p56 (0061.jp2)
- CHAPTER5:Cloning and Sequencing of CLS9, CLS10 and CLS11 Genes / p73 (0078.jp2)
- 5.1 Introduction / p73 (0078.jp2)
- 5.2 Results / p74 (0079.jp2)
- 5.3 Discussion / p77 (0082.jp2)
- CHAPTER6:Characterization of cls9 Disrupted Cell / p89 (0094.jp2)
- 6.1 Introduction / p89 (0094.jp2)
- 6.2 Results / p90 (0095.jp2)
- 6.3 Discussion / p92 (0097.jp2)
- Appendix:Cloning and Disruption of VMA2 gene / p103 (0108.jp2)
- A.1 Cloning and sequence of VMA2 gene / p103 (0108.jp2)
- A.2 Disruption of VMA2 gene / p103 (0108.jp2)
- REFERENCE / p108 (0113.jp2)