Studies on a short-term bioassay of toxic chemicals using Saccharomyces cerevisiae Saccharomyces cerevisiaeを用いた有害化学物質の短期間生物試験に関する研究

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Author

    • Athanassios Kungolos アタナシオス クンゴロス

Bibliographic Information

Title

Studies on a short-term bioassay of toxic chemicals using Saccharomyces cerevisiae

Other Title

Saccharomyces cerevisiaeを用いた有害化学物質の短期間生物試験に関する研究

Author

Athanassios Kungolos

Author(Another name)

アタナシオス クンゴロス

University

岡山大学

Types of degree

学術博士

Grant ID

甲第1027号

Degree year

1992-03-28

Note and Description

博士論文

Table of Contents

  1. CONTENTS / p1 (0003.jp2)
  2. SUMMARY / p5 (0007.jp2)
  3. A.INTRODUCTION / p9 (0011.jp2)
  4. A.1.Purpose of the study. / p9 (0011.jp2)
  5. A.2.Toxicity tests in general. / p11 (0013.jp2)
  6. A.3.Mutagenicity tests (Ames test, Rec assay etc.) / p11 (0013.jp2)
  7. A.4.Earlier use of Saccharomyces cerevisiae in toxicity testing. / p14 (0016.jp2)
  8. A.5.Advantages and disadvantages of S.cerevisiae as a test organism. / p14 (0016.jp2)
  9. B.MATERIALS AND METHODS / p16 (0018.jp2)
  10. B.1.Culture of S.cerevisiae. / p16 (0018.jp2)
  11. B.2.Different strains of S.cerevisiae. / p17 (0019.jp2)
  12. B.3.Bioscreen-C analyzer system. / p19 (0021.jp2)
  13. B.4.Relationship between light absorbance and cell density. / p22 (0024.jp2)
  14. B.5.Mathematical model for dose-response. / p24 (0026.jp2)
  15. B.6.Methods used for the evaluation of the interactive effect of chemicals. / p25 (0027.jp2)
  16. B.7.Statistical analysis of the results. / p27 (0029.jp2)
  17. C.ANALYSIS OF THE GROWTH OF S.CEREVISIAE IN THE PRESENCE OF CHEMICALS / p30 (0032.jp2)
  18. C.1.Indices used for toxicity testing. / p30 (0032.jp2)
  19. C.2.Survival rate of S.cerevisiae. / p32 (0034.jp2)
  20. C.3.Maximum specific growth rate. / p33 (0035.jp2)
  21. C.4.Area till a given point. / p36 (0038.jp2)
  22. C.5.Maximum absorbance and maximum cell density. / p37 (0039.jp2)
  23. C.6.Turning point of the growth curve. / p38 (0040.jp2)
  24. D.EFFECT OF VARIOUS FORMS OF MERCURY ON S.CEREVISIAE / p40 (0042.jp2)
  25. D.1.Effect of organic mercury. / p40 (0042.jp2)
  26. D.2.Effect of inorganic forms. / p41 (0043.jp2)
  27. D.3.Comparison between different kinds of strains. / p45 (0047.jp2)
  28. D.4.Bioaccumulation of Hg in the cells of S. cercvisiae. / p47 (0049.jp2)
  29. D.5.Relationship between bioaccumulation and toxicity. / p50 (0052.jp2)
  30. D.6.Effect of complexing and chelating agents on the toxicity of Hg to S.cerevisiae. / p53 (0055.jp2)
  31. E.EFFECTS OF VARIOUS HEAVY METALS ON S.CEREVISIAE / p56 (0058.jp2)
  32. E.1.Effect of cadmium and nickel. / p56 (0058.jp2)
  33. E.2.Comparison between trivalent and hexavalent chromium. / p58 (0060.jp2)
  34. E.3.Effects of zinc, lead and copper. / p59 (0061.jp2)
  35. E.4.Using EDTA in order to avoid the precipitation problem. / p61 (0063.jp2)
  36. F.INTERACTIVE EFFECTS OF CHEMICALS / p63 (0065.jp2)
  37. F.1.Suggesting a method for evaluating the interactive effect of the chemicals. / p63 (0065.jp2)
  38. F.2.Theoretical basis for the "independent events method". / p64 (0066.jp2)
  39. F.3.Interactive effect of two chemicals. / p65 (0067.jp2)
  40. F.4.Interactive effect of three chemicals. / p67 (0069.jp2)
  41. F.5.Interactive effect of more than three chemicals. / p69 (0071.jp2)
  42. F.6.Interactive effect between chromium and nickel. / p70 (0072.jp2)
  43. F.7.Interactive effect between nickel and cadmium. / p72 (0074.jp2)
  44. F.8.Interactive effect between cadmium and chromium. / p74 (0076.jp2)
  45. F.9.Interactive effect between chromium, cadmium and nickel. / p75 (0077.jp2)
  46. G.EFFECTS OF ORGANIC CHEMICALS ON S.CEREVISIAE / p80 (0082.jp2)
  47. G.1.Ethyl alcohol as a solvent. / p80 (0082.jp2)
  48. G.2.DMSO and acetone as solvents. / p82 (0084.jp2)
  49. G.3.Effect of monochlorophenols. / p84 (0086.jp2)
  50. G.4.Effect of dichlorophenols. / p87 (0089.jp2)
  51. G.5.Effect of trichlorophenols. / p89 (0091.jp2)
  52. G.6.Effect of tetrachlorophenol. / p91 (0093.jp2)
  53. G.7.Effect of pentachlorophenol. / p93 (0095.jp2)
  54. G.8.Interactive effect of chlorophenols. / p94 (0096.jp2)
  55. G.9.Rationale for using QSAR. / p95 (0097.jp2)
  56. G.10.QSAR's for the effect of chlorophenols on S.cerevisiae. / p97 (0099.jp2)
  57. H.TESTING DNA DAMAGE CAUSED BY CHEMICALS / p101 (0103.jp2)
  58. H.1.Principle of the method. / p101 (0103.jp2)
  59. H.2.Comparison with other short-term mutagenicity and DNA damage assays. / p102 (0104.jp2)
  60. H.3.The effect of 4NQO. / p104 (0106.jp2)
  61. H.4.The effect of kanamycin. / p107 (0109.jp2)
  62. H.5.DNA damage caused by chlorophenols. / p108 (0110.jp2)
  63. H.6.DNA damage caused by Hg. / p110 (0112.jp2)
  64. H.7.The effect of bromouracil. / p111 (0113.jp2)
  65. H.8.The effect of nicotinic acid. / p113 (0115.jp2)
  66. I.CONCLUSIONS / p115 (0117.jp2)
  67. I.1.Evaluation of the method when used for toxieity assessment. / p115 (0117.jp2)
  68. I.2.Evaluation of the method when used for testing DNA damage. / p116 (0118.jp2)
  69. I.3.Future expansion of this study. / p116 (0118.jp2)
  70. LITERATURE CITED / p118 (0120.jp2)
  71. ACKNOWLEDGEMENTS / p129 (0131.jp2)
8access

Codes

  • NII Article ID (NAID)
    500000083862
  • NII Author ID (NRID)
    • 8000000084072
  • DOI(NDL)
  • Text Lang
    • jpn
  • NDLBibID
    • 000000248176
  • Source
    • Institutional Repository
    • NDL ONLINE
    • NDL Digital Collections

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