Molecular cloning and expression of human and mouse medullasin/leukocyte elastase cDNA ヒトおよびマウスのメダラシン/白血球エラスターゼcDNAのクローニングと発現

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Author

    • 岡野, 清 オカノ, キヨシ

Bibliographic Information

Title

Molecular cloning and expression of human and mouse medullasin/leukocyte elastase cDNA

Other Title

ヒトおよびマウスのメダラシン/白血球エラスターゼcDNAのクローニングと発現

Author

岡野, 清

Author(Another name)

オカノ, キヨシ

University

九州大学

Types of degree

理学博士

Grant ID

乙第5090号

Degree year

1992-01-30

Note and Description

博士論文

Table of Contents

  1. A TABLE OF CONTENTS / p2 (0003.jp2)
  2. SUMMARY / p4 (0005.jp2)
  3. INTRODUCTION / p6 (0007.jp2)
  4. MATERIALS AND METHODS / p9 (0010.jp2)
  5. 1.Materials / p9 (0010.jp2)
  6. 2.Peptide sequencing / p9 (0010.jp2)
  7. 3.Medullasin assay / p10 (0011.jp2)
  8. 4.Northern blot and mRNA analysis / p10 (0011.jp2)
  9. 5.Southern blot and chromosome analysis / p11 (0012.jp2)
  10. 6.Construction of a cDNA library / p11 (0012.jp2)
  11. 7.Screening of a cDNA library / p12 (0013.jp2)
  12. 8.DNA sequencing and DNA sequence analysis / p13 (0014.jp2)
  13. 9.Construction and screening of a human genomic gene library / p13 (0014.jp2)
  14. 10.Expression of cDNAs in COS-1 cells. / p13 (0014.jp2)
  15. 11.Elastase assay / p14 (0015.jp2)
  16. RESULTS / p15 (0016.jp2)
  17. 1.Partial amino acid sequence of medullasin / p15 (0016.jp2)
  18. 2.Screening of human leukemia cell lines with medullasin activity / p15 (0016.jp2)
  19. 3.Molecular cloning of medullasin cDNA / p18 (0019.jp2)
  20. 4.Expression of recombinant medullasin/HLE / p20 (0021.jp2)
  21. 5.Northern blot analysis of human leukemia cell lines with medullasin cDNA probe / p28 (0029.jp2)
  22. 6.Molecular cloning of medullasin/HLE gene / p28 (0029.jp2)
  23. 7.Molecular cloning of mouse leukocyte elastase (MLE) cDNA / p35 (0036.jp2)
  24. 8.Deduced amino acid sequence of MLE and sequence comparison to medullasin/HLE / p35 (0036.jp2)
  25. 9.Biological characterization of MLE / p40 (0041.jp2)
  26. DISCUSSION / p47 (0048.jp2)
  27. 1.Medullasin is a human leukocyte elastase (HLE) / p47 (0048.jp2)
  28. 2.Structure of medullasin/HLE precursor and its processing / p47 (0048.jp2)
  29. 3.Sequence homology among medullasin/HLE and other serine proteases / p48 (0049.jp2)
  30. 4.Expression of medullasin/HLE mRNA in human leukemia cell lines / p52 (0053.jp2)
  31. 5.Comparison of exon-intron structure with other serine protease genes / p53 (0054.jp2)
  32. 6.WEHI-3 cDNA clone is considered to be a mouse leukocyte elastase / p53 (0054.jp2)
  33. 7.New expression system for an intracellular protease as a secreted product / p54 (0055.jp2)
  34. ACKNOWLEDGEMENTS / p56 (0057.jp2)
  35. REFERENCES / p57 (0058.jp2)
7access

Codes

  • NII Article ID (NAID)
    500000084809
  • NII Author ID (NRID)
    • 8000000085021
  • DOI(NDL)
  • NDLBibID
    • 000000249123
  • Source
    • NDL ONLINE
    • NDL Digital Collections

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