Studies on the cryopreservation of mouse embryos by vitrification マウス胚のガラス化による低温保存に関する研究

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Author

    • Conrado A. Valdez コンラド アントニオ バルデス

Bibliographic Information

Title

Studies on the cryopreservation of mouse embryos by vitrification

Other Title

マウス胚のガラス化による低温保存に関する研究

Author

Conrado A. Valdez

Author(Another name)

コンラド アントニオ バルデス

University

北海道大学

Types of degree

獣医学博士

Grant ID

甲第3079号

Degree year

1992-03-25

Note and Description

博士論文

Table of Contents

  1. CONTENTS / (0003.jp2)
  2. GENERAL INTRODUCTION / p1 (0006.jp2)
  3. PART 1 STUDIES ON SOME FACTORS AFFECTING THE SURVIVAL OF MOUSE EMBRYOS CRYOPRESERVED BY VITRIFICATION / p6 (0011.jp2)
  4. CHAPTER 1 EFFECTS OF EQUILIBRATION TIME,PRECOOLING AND DEVELOPMENTAL STAGE ON THE SURVIVAL OF MOUSE EMBRYOS CRYOPRESERVED BY VITRIFICATION / p7 (0012.jp2)
  5. Introduction / p7 (0012.jp2)
  6. Materials and Methods / p8 (0013.jp2)
  7. Results / p13 (0018.jp2)
  8. Discussion / p16 (0021.jp2)
  9. Summary / p20 (0025.jp2)
  10. CHAPTER 11 IN VITRO AND IN VIVO DEVELOPMENT OF VITRIFIED-WARMED MOUSE MORULAE / p21 (0026.jp2)
  11. Introduction / p21 (0026.jp2)
  12. Materials and Methods / p22 (0027.jp2)
  13. 1.In vitro experiment / p23 (0028.jp2)
  14. 2.In vivo experiment / p24 (0029.jp2)
  15. Results / p25 (0030.jp2)
  16. Discussion / p28 (0033.jp2)
  17. Summary / p31 (0036.jp2)
  18. CHAPTER 111 CONFIRMATION BY CRYO-ELECTRON MICROSCOPY OF THE ABSENCE OF CRYSTALLIZATION USING A VITRIFICATION SOLUTION / p32 (0037.jp2)
  19. Introduction / p32 (0037.jp2)
  20. Materials and Methods / p33 (0038.jp2)
  21. 1.Light microscopic examination / p34 (0039.jp2)
  22. 2.Cryo-SEM & Freeze fracture EM examination / p35 (0040.jp2)
  23. Results / p35 (0040.jp2)
  24. Discussion / p40 (0045.jp2)
  25. Summary / p43 (0048.jp2)
  26. PART 11 STUDIES ON THE DEVELOPMENT OF A NEW VITRIFICATION SOLUTION / p44 (0049.jp2)
  27. CHAPTER 1V EFFECTS OF VARIOUS CRYOPROTECTANTS ON THE SURVIVAL OF NONFROZEN MOUSE BLASTOCYSTS / p45 (0050.jp2)
  28. Introduction / p45 (0050.jp2)
  29. Materials and Methods / p46 (0051.jp2)
  30. 1.Effect of cryoprotectant concentration / p46 (0051.jp2)
  31. 2.Effect of length of exposure / p47 (0052.jp2)
  32. 3.Effect of cryoprotectant dilution / p48 (0053.jp2)
  33. Results / p49 (0054.jp2)
  34. Discussion / p55 (0060.jp2)
  35. Summary / p60 (0065.jp2)
  36. CHAPTER V SUCCESSFUL CRYOPRESERVATION OF MOUSE BLASTOCYSTS USING A NEW VITRIFICATION SOLUTION / p61 (0066.jp2)
  37. Introduction / p61 (0066.jp2)
  38. Materials and Methods / p62 (0067.jp2)
  39. 1.In vitro experiment / p64 (0069.jp2)
  40. 2.In vivo experiment / p65 (0070.jp2)
  41. Results / p67 (0072.jp2)
  42. Discussion / p71 (0076.jp2)
  43. Summary / p79 (0084.jp2)
  44. CHAPTER V1 ULTRASTRUCTURAL OBSERVATIONS OF MOUSE BLASTOCYSTS AFTER EXPOSURE IN VITRIFICATION SOLUTION / p80 (0085.jp2)
  45. Introduction / p80 (0085.jp2)
  46. Materials and Methods / p80 (0085.jp2)
  47. 1.Light microscopic examination / p81 (0086.jp2)
  48. 2.Freeze fracture EM examination / p82 (0087.jp2)
  49. Results / p82 (0087.jp2)
  50. Discussion / p87 (0092.jp2)
  51. Summary / p90 (0095.jp2)
  52. GENERAL CONCLUSIONS / p102 (0107.jp2)
  53. SUMMARY / p105 (0110.jp2)
  54. SUMMARY IN JAPANESE / p108 (0113.jp2)
  55. ACKNOWLEDGEMENTS / p111 (0116.jp2)
  56. REFERENCES / p112 (0117.jp2)
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Codes

  • NII Article ID (NAID)
    500000085315
  • NII Author ID (NRID)
    • 8000000085529
  • DOI(NDL)
  • NDLBibID
    • 000000249629
  • Source
    • NDL ONLINE
    • NDL Digital Collections

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