Studies on overcoming breeding barriers between Lycopersicon esculentum and the "peruvianum-complex" by means of biotechnology バイオテクノロジー手法を用いたトマト種間における育種障害克服に関する研究

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Title: Studies on overcoming breeding barriers between Lycopersicon esculentum and the "peruvianum-complex" by means of biotechnology

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Thesis

Contents / (0003.jp2)
1.Introduction / p1 (0006.jp2)
2.Histological studies on the embryo development of the interspecific hybrids and backcrosses by Nomarski's differential interference microscopy / p7 (0012.jp2)
2.1 Introduction / p7 (0012.jp2)
2.2 The process of embryo collapse in the interspecific hybrids / p9 (0014.jp2)
2.3.Postfertilization hybrid ovule abortion in backcrosses to L.esculentum / p22 (0027.jp2)
3.Rescue of hybrid embryos and clonal propagation / p32 (0037.jp2)
3.1 Introduction / p32 (0037.jp2)
3.2 Interspecific hybridization by in oitro ovule culture / p33 (0038.jp2)
3.3 Efficient embryo culture and clonal propagation of hybrids / p45 (0050.jp2)
3.4 Efficiency of embryo culture in the backcrosses / p58 (0063.jp2)
4.improvement of protoplast culture and regeneration from cotyledon protoplasts of tomato / p72 (0077.jp2)
4.1 Introduction / p72 (0077.jp2)
4.2 Effects of pretreatments on the yield and fate of protoplasts / p74 (0079.jp2)
4.3 Regeneration from cotyledon protoplasts of L.esculentum cv.Kyoryokutoko / p93 (0098.jp2)
5.Somatic hybridization betweenl L.esculentum and the＇peruoianum-complex＇ / p106 (0111.jp2)
5.1 Introduction / p106 (0111.jp2)
5.2 Regeneration from somatic hybridization of L.esculentum and the＇peruoianum-comp lex＇ / p109 (0114.jp2)
5.3 Identification of somatic hybrid calli by RFLP / p118 (0123.jp2)
6.Conclusion / p125 (0130.jp2)
7.Acknowledgements / p135 (0140.jp2)
8.References / p137 (0142.jp2)

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