Probing the active site in UDP-glucose pyrophosphorylase by affinity labeling

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Title: Probing the active site in UDP-glucose pyrophosphorylase by affinity labeling

博士論文

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CONTENTS / (0006.jp2)
List of Abbreviations / p1 (0007.jp2)
Chapter I General Introduction / p2 (0009.jp2)
Figures / p11 (0018.jp2)
References / p13 (0020.jp2)
Chapter II Identification of Lysyl Residues Located at the Substrate-Binding Site: Affinity Labeling with Uridine Di- and Triphosphopyridoxals / p17 (0025.jp2)
Introduction / p17 (0025.jp2)
Experimental Procedures / p18 (0026.jp2)
Results / p23 (0031.jp2)
Disscussion / p28 (0036.jp2)
Tables and Figures / p32 (0040.jp2)
References / p39 (0047.jp2)
Chapter III Comparative Affinity Labeling with Reactive UDP-Glucose Analogues: Possible Locations of Five Lysyl Residues / p41 (0050.jp2)
Introduction / p41 (0050.jp2)
Experimental Procedures / p43 (0052.jp2)
Results / p46 (0055.jp2)
Disscussion / p51 (0060.jp2)
Tables and Figures / p56 (0065.jp2)
References / p64 (0073.jp2)
Chapter IV Probing the Pyrophosphate-Binding Site by Affinity Labeling with Pyridoxal Diphosphate / p66 (0076.jp2)
Introduction / p66 (0076.jp2)
Experimental Procedures / p67 (0077.jp2)
Results / p70 (0080.jp2)
Disscussion / p76 (0086.jp2)
Figures / p81 (0091.jp2)
References / p87 (0097.jp2)
Chapter V Comprehensive Discussion / p88 (0099.jp2)
Table and Figure / p92 (0103.jp2)
References / p95 (0106.jp2)
Acknowledgement / p96 (0107.jp2)

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Probing the active site in UDP-glucose pyrophosphorylase by affinity labeling 親和標識によるUDP-グルコース・ピロホスホリラーゼ活性部位の構造解析