Complete assignment of proton, carbon 13, and nitrogen 15 resonance peaks in large proteins with three-dimensional nuclear magnetic resonance spectroscopies

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タイトル: Complete assignment of proton, carbon 13, and nitrogen 15 resonance peaks in large proteins with three-dimensional nuclear magnetic resonance spectroscopies

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CONTENTS / (0003.jp2)
I.INTRODUCTION / p1 (0007.jp2)
1.General introduction / p1 (0007.jp2)
2.NMR spectroscopy / p4 (0009.jp2)
3.NMR of proteins / p9 (0011.jp2)
4.2D NMRs used as components of 3D NMR experiments / p15 (0014.jp2)
5.Opening of 3D NMR / p19 (0016.jp2)
6.Ribonuclease H / p27 (0020.jp2)
II.THREE DIMENSIONAL NMR / p40 (0028.jp2)
1.Introduction / p40 (0028.jp2)
2.ID,2D,and 3D NMR / p40 (0028.jp2)
3.NMR apparatus / p42 (0029.jp2)
4.Principles of r.f.pulse and Fourier transformation / p44 (0030.jp2)
5.Pulse sequences / p53 (0035.jp2)
6.Data processing / p64 (0040.jp2)
III.ISOTOPE LABELING / p83 (0051.jp2)
1.Introduction / p83 (0051.jp2)
2.Overexpression system / p83 (0051.jp2)
3.Sample preparation / p84 (0052.jp2)
4.Uniform labeling / p86 (0053.jp2)
5.Amino acid selective labeling / p87 (0053.jp2)
6.Correlation labeling / p88 (0054.jp2)
IV.ASSIGNMENT OF BACKBONE NUCLEI / p97 (0059.jp2)
1.Introduction / p97 (0059.jp2)
2.Assignment strategy / p97 (0059.jp2)
3.Examples of assignment with ribonuclease H / p102 (0062.jp2)
V.ASSIGNMENT OF SIDECHAIN NUCLEI / p128 (0076.jp2)
1.Introduction / p128 (0076.jp2)
2.Assignment strategy / p128 (0076.jp2)
3.Examples of assignment with ribonuclease H / p130 (0077.jp2)
VI.RESULTS AND DISCUSSION ON ASSIGNMENTS OF NUCLEI IN RIBONUCLEASE H / p157 (0092.jp2)
1.Introduction / p157 (0092.jp2)
2.Results of complete assignments / p157 (0092.jp2)
3.Interpretation of the obtained complete assignments and NMR parameters / p159 (0093.jp2)
VII.CONCLUSION / p191 (0110.jp2)