Biochemical and molecular aspects of bovine C-reactive protein ウシC-反応性タンパク質の生化学的および分子生物学的性状
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著者
書誌事項
- タイトル
-
Biochemical and molecular aspects of bovine C-reactive protein
- タイトル別名
-
ウシC-反応性タンパク質の生化学的および分子生物学的性状
- 著者名
-
Sarikaputi, Meena
- 著者別名
-
サリカプティ, ミーナ
- 学位授与大学
-
北海道大学
- 取得学位
-
博士 (獣医学)
- 学位授与番号
-
乙第4195号
- 学位授与年月日
-
1992-12-25
注記・抄録
博士論文
目次
- CONTENTS / p1 (0003.jp2)
- Abbreviations / p4 (0006.jp2)
- Preface / p1 (0008.jp2)
- Chapter 1 Purification of bovine C-reactive protein and serum amyloid P component / p4 (0011.jp2)
- Introduction / p4 (0011.jp2)
- Materials and Methods / p4 (0011.jp2)
- Materials / p4 (0011.jp2)
- Purification of bovine CRP and SAP / p5 (0012.jp2)
- Protein purity analysis / p6 (0013.jp2)
- Determination of disulfide bond / p6 (0013.jp2)
- Quantitative determination of CRP and SAP / p6 (0013.jp2)
- Results / p7 (0014.jp2)
- Discussion / p11 (0018.jp2)
- Summary / p13 (0020.jp2)
- Chapter 2 Latex agglutination test : A simple, rapid and practical method for bovine serum C-reactive protein determination / p14 (0021.jp2)
- Introduction / p14 (0021.jp2)
- Materials and Methods / p14 (0021.jp2)
- Materials / p14 (0021.jp2)
- Preparation of latex particle suspension / p15 (0022.jp2)
- Purification of anti-bovine CRP antibody / p15 (0022.jp2)
- Coating of latex particles with purified Ab / p16 (0023.jp2)
- Latex agglutination test / p16 (0023.jp2)
- Reproducibility of the assay / p17 (0024.jp2)
- Analysis of the recovery assay / p17 (0024.jp2)
- Correlation of latex agglutination test with SRID test / p17 (0024.jp2)
- Effects of storage at 4°C and freezing-thawing on CRP assay / p17 (0024.jp2)
- Results / p18 (0025.jp2)
- Discussion / p27 (0034.jp2)
- Summary / p30 (0037.jp2)
- Chapter 3 Production of monoclonal antibodies against bovine C-reactive protein / p31 (0038.jp2)
- Introduction / p31 (0038.jp2)
- Materials and Methods / p31 (0038.jp2)
- Materials / p31 (0038.jp2)
- Immunization / p32 (0039.jp2)
- Antibody screening procedure / p32 (0039.jp2)
- Fusion and hybridoma production / p33 (0040.jp2)
- Ascitic fluid production / p34 (0041.jp2)
- Purification of MAbs / p34 (0041.jp2)
- Class and subclass identification of MAbs / p34 (0041.jp2)
- Antibody titer and concentration / p34 (0041.jp2)
- Cross-reactivity with CRP of other species / p35 (0042.jp2)
- Results / p35 (0042.jp2)
- Discussion / p40 (0047.jp2)
- Summary / p41 (0048.jp2)
- Chapter 4 Partial sequence of bovine C-reactive protein complementary DNA / p43 (0050.jp2)
- Introduction / p43 (0050.jp2)
- Materials and Methods / p43 (0050.jp2)
- Materials / p43 (0050.jp2)
- Preparation of DNA probe for bovine CRP by polymerase chain reaction (PCR) / p44 (0051.jp2)
- Bovine liver DNA and RNA isolation / p44 (0051.jp2)
- Cloning of bovine CRP cDNA / p44 (0051.jp2)
- Restriction map of bovine CRP cDNA / p46 (0053.jp2)
- DNA sequencing / p46 (0053.jp2)
- Results / p46 (0053.jp2)
- Discussion / p50 (0057.jp2)
- Summary / p52 (0059.jp2)
- Conclusions in English / p54 (0061.jp2)
- Conclusions in Japanese / p56 (0063.jp2)
- References / p58 (0065.jp2)
- Acknowledgement. / p68 (0075.jp2)