Studies of fission yeast homologues of a RAS protein-processing gene of baker's yeast Studies of Fission Yeast Homologues of a RAS Protein-Processing Gene of Baker's Yeast

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Title: Studies of fission yeast homologues of a RAS protein-processing gene of baker's yeast

Other Title: Studies of Fission Yeast Homologues of a RAS Protein-Processing Gene of Baker's Yeast

博士論文

総研大甲第38号

Contents / p2 (0003.jp2)
Genetic Nomenclature of Yeast / p4 (0005.jp2)
Abstract / p5 (0006.jp2)
Introduction / p6 (0007.jp2)
Materials and Methods / p9 (0010.jp2)
Strains. / p9 (0010.jp2)
Plasmids. / p9 (0010.jp2)
Media. / p10 (0011.jp2)
Plasmid DNA Preparation from E.coli. / p11 (0012.jp2)
Genomic DNA Preparation from S.pombe. / p13 (0014.jp2)
Southern Blot analysis. / p14 (0015.jp2)
Construction of S.pombe Genomic DNA Library. / p14 (0015.jp2)
Plaque Hybridization. / p15 (0016.jp2)
RNA Preparation from S.pombe. / p15 (0016.jp2)
Northern Blot Analysis. / p16 (0017.jp2)
Construction of S.pombe cDNA Library. / p16 (0017.jp2)
Transformation of S.pombe Cells. / p17 (0018.jp2)
Gene Disruption. / p17 (0018.jp2)
Random Spore Analysis. / p18 (0019.jp2)
DNA Sequence Analysis. / p18 (0019.jp2)
PCR Amplification of S.pombe cDNA. / p20 (0021.jp2)
Results. / p21 (0022.jp2)
Isolation of Four pdh (S.pombe DPR1 homologous clone)Clones from the S.pombe Genomic DNA Library. / p21 (0022.jp2)
Detection of the RNA Transcripts from the Four pdh Locus of S.pombe. / p24 (0025.jp2)
Genetic Characterization of pdh1 Locus. / p25 (0026.jp2)
The Nucleotide Sequence and Deduced Amino Acid Sequence of S.pombe cDNA clone pdh1c10A-1. / p28 (0029.jp2)
Sequence Analysis of the pdh1 and pdh2 Genomic DNA. / p30 (0031.jp2)
PCR Amplification of DPR1 Related Genes from S.pombe cDNA Library. / p35 (0036.jp2)
Discussion. / p38 (0039.jp2)
Acknowledgments. / p42 (0043.jp2)
References / p43 (0044.jp2)

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