Molecular study of leukocidin gene from methicillin resistant Staphylococcus aureus メチシリン耐性黄色ブドウ球菌のロイコシジン遺伝子に関する研究
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著者
書誌事項
- タイトル
-
Molecular study of leukocidin gene from methicillin resistant Staphylococcus aureus
- タイトル別名
-
メチシリン耐性黄色ブドウ球菌のロイコシジン遺伝子に関する研究
- 著者名
-
Rahman, Arifur
- 著者別名
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ラーマン, アリフール
- 学位授与大学
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東北大学
- 取得学位
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博士 (農学)
- 学位授与番号
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甲第4892号
- 学位授与年月日
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1993-03-25
注記・抄録
博士論文
目次
- CONTENTS / p1 (0005.jp2)
- LIST OF FIGURES / p13 (0017.jp2)
- LIST OF CHART / p17 (0021.jp2)
- LIST OF TABLES / p18 (0022.jp2)
- CHAPTER:INTRODUCTION / p1 (0024.jp2)
- 1.1 GENERAL INTRODUCTION / p1 (0024.jp2)
- 1.2.METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS / p7 (0030.jp2)
- CHAPTER:MATERIALS AND METHODS / p11 (0034.jp2)
- 2.1.LABORATORY APPARATUS / p11 (0034.jp2)
- 2.2.LABORATORY REAGENTS / p11 (0034.jp2)
- 2.3.STOCK SOLUTIONS / p12 (0035.jp2)
- 2.4.MICROBIOLOGICAL MEDIA AND PLATES / p18 (0041.jp2)
- 2.5.DETECTION OF LEUKOCIDIN BY USING POLYCLONAL ANTIBODY / p20 (0043.jp2)
- 2.6.SCREENING FOR THE PRESENCE OF LEUKOCIDIN IN MRSA / p28 (0051.jp2)
- 2.7.PURIFICATION OF LEUKOCIDIN / p29 (0052.jp2)
- 2.8.LEUKOCYTOLYTIC ACTIVITY OF PURIFIED S AND F-COMPONENTS FROM MRSA NUMBER 4 STRAIN. / p32 (0055.jp2)
- 2.9.ISOLATION OF CHROMOSOMAL DNA FROM MRSA STRAIN / p34 (0058.jp2)
- 2.10.ISOLATION OF PLASMID AND COSMID DNA / p37 (0061.jp2)
- 2.11.QUANTITATIVE DETERMINATION OF NUCLEIC ACID / p44 (0068.jp2)
- 2.12.DIGESTION OF DNA WITH RESTRICTION ENZYMES / p45 (0069.jp2)
- 2.13.SYNTHESIS OF OLIGONUCLEOTIDE PROBES / p46 (0070.jp2)
- 2.14.TRANSFORMATION OF BACTERIA / p52 (0076.jp2)
- 2.15.SIZE FRACTIONATION USING SUCROSE GRADIENTS / p56 (0080.jp2)
- 2.16.ELECTROPHORESIS OF DNA / p60 (0084.jp2)
- 2.17.EXTRACTION OF DNA FRAGMENTS FROM AGAROSE GELS / p61 (0085.jp2)
- 2.18.BLOTTING OF DNA / p62 (0086.jp2)
- 2.19.RADIOLABELING OF SYNTHETIC OLIGONUCLEOTIDE PROBES / p64 (0088.jp2)
- 2.20.RANDOM PRIMED DNA LABELING / p66 (0090.jp2)
- 2.21.HYBRIDIZATION OF DNA / p68 (0092.jp2)
- 2.22.SCREENING OF THE GENOMIC LIBRARY / p72 (0096.jp2)
- 2.23.CLONING OF DNA FRAGMENTS / p74 (0098.jp2)
- 2.24.IN VITRO TRANSCRIPTION/TRANSLATION ASSAY / p81 (0105.jp2)
- 2.25.IN VIVO PROTEIN EXPRESSION / p84 (0108.jp2)
- 2.26.CELLULAR LOCALIZATION OF EXPRESSED PROTEIN / p85 (0109.jp2)
- 2.27.SEQUENCING OF DNA / p86 (0110.jp2)
- CHAPTER:RESULTS AND DISCUSSION / p99 (0123.jp2)
- 3.1.SCREENING FOR THE PRESENCE OF LEUKOCIDIN IN MRSA / p99 (0123.jp2)
- 3.2.PURIFICATION OF S AND F-COMPONENTS FROM MRSA NO.4 STRAIN / p100 (0124.jp2)
- 3.3.LEUKOCYTOLYTIC ACTIVITY OF THE PURIFIED COMPONENTS OF LEUKOCIDIN FROM MRSA NO.4 STRAIN / p106 (0131.jp2)
- 3.4.N-TERMINAL AMINO ACID SEQUENCE / p106 (0131.jp2)
- 3.5.CLONING OF S-COMPONENT GENE IN ESCHERICHIA COLI DH5α / p110 (0135.jp2)
- 3.6.CLONING OF F-COMPONENT GENE IN ESCHERICHIA COLI DH5α / p135 (0162.jp2)
- 3.7.LEUKOCYTOLYTIC ACTIVITY OF CLONED S AND F-COMPONENTS / p146 (0174.jp2)
- CHAPTER:GENERAL DISCUSSION / p151 (0180.jp2)
- 4.0.CLONING OF LEUKOCIDIN GENE / p151 (0180.jp2)
- 4.1.CODON USAGE / p153 (0182.jp2)
- 4.2.HYDROPHOBICITY OF S AND F-COMPONENTS / p156 (0185.jp2)
- 4.3.HOMOLOGY STUDY BETWEEN S AND F-COMPONENTS / p156 (0185.jp2)
- 4.4.RELATION BETWEEN LEUKOCIDIN AND GAMMA-HEMOLYSIN / p156 (0185.jp2)
- 4.5.CONCLUSION AND FUTURE ASPECTS OF STUDY / p159 (0188.jp2)
- CHAPTER:REFERENCES / p162 (0191.jp2)