Biotransformation of benzene into cis-benzene glycol by a genetically modified Pseudomonas Pseudomonasによるシス-ベンゼンのベンゼングリコールへの転換

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著者

    • Yarmoff, Jean-Jacques ヤルモフ, ジャン ジャック

書誌事項

タイトル

Biotransformation of benzene into cis-benzene glycol by a genetically modified Pseudomonas

タイトル別名

Pseudomonasによるシス-ベンゼンのベンゼングリコールへの転換

著者名

Yarmoff, Jean-Jacques

著者別名

ヤルモフ, ジャン ジャック

学位授与大学

東京大学

取得学位

博士 (工学)

学位授与番号

乙第10505号

学位授与年月日

1992-01-23

注記・抄録

博士論文

目次

  1. 論文の内容の要旨 / (0003.jp2)
  2. Table of Contents / p2 (0007.jp2)
  3. Introduction. / p1 (0011.jp2)
  4. Part1.Characterization of a cis-benzene glycol producing strain. / p3 (0013.jp2)
  5. Chapter1.Biological oxidation of aromatic hydrocarbons. / p3 (0013.jp2)
  6. 1.1.Metabolism of aromatic hydrocarbons in eukaryotes. / p3 (0013.jp2)
  7. 1.2.Metabolism of hydrocarbons in prokaryotes. / p10 (0020.jp2)
  8. 1.3.Anaerobic degradation of aromatic compounds. / p25 (0035.jp2)
  9. 1.4.Conclusions. / p28 (0038.jp2)
  10. Chapter2.Strain isolation. / p29 (0039.jp2)
  11. 2.1.Aromatics and benzene degraders. / p29 (0039.jp2)
  12. 2.2.Growth of bacteria with benzene as carbon source: apparatus and media. / p29 (0039.jp2)
  13. 2.3.Isolation of benzene degraders. / p33 (0043.jp2)
  14. 2.4.Strain choice and preliminary identification. / p35 (0045.jp2)
  15. 2.5.Utilization of various carbon sources by the strain JI104. / p40 (0050.jp2)
  16. 2.6.Characterization of the strain Pseudomonas JI104. / p40 (0050.jp2)
  17. 2.7.Conclusions. / p46 (0055.jp2)
  18. Chapter3.Localization of the benzene genes. / p47 (0056.jp2)
  19. 3.1.Benzene degraders. / p47 (0056.jp2)
  20. 3.2.Stability of the benzene degradation pathway of Pseudomonas JI104. / p48 (0057.jp2)
  21. 3.3.Plasmid isolation. / p49 (0058.jp2)
  22. 3.4.Conjugation to other strains. / p52 (0061.jp2)
  23. 3.5.Cloning attempts. / p54 (0063.jp2)
  24. 3.6.Conclusions. / p55 (0064.jp2)
  25. Chapter4.Gene tagging and transposon mutagenesis. / p56 (0065.jp2)
  26. 4.1.Transposon mutagenesis:interest. / p56 (0065.jp2)
  27. 4.2.Targets of the transposon mutation. / p57 (0066.jp2)
  28. 4.3.Screening methods. / p58 (0067.jp2)
  29. 4.4.Transposition mutagenesis systems. / p60 (0069.jp2)
  30. 4.5.Optimization of the system. / p66 (0075.jp2)
  31. 4.6.Mutants isolation. / p71 (0080.jp2)
  32. 4.7.Mutants characterization. / p72 (0081.jp2)
  33. 4.8.Effect of benzoate as inducer. / p77 (0086.jp2)
  34. 4.9.Conclusions. / p78 (0087.jp2)
  35. Chapter5.Chemical mutagenesis. / p79 (0088.jp2)
  36. 5.1.Mutation method. / p79 (0088.jp2)
  37. 5.2.Mutants isolation and characterization. / p81 (0091.jp2)
  38. 5.3.Identification of cis-benzene glycol. / p86 (0096.jp2)
  39. 5.4.cis-Benzene glycol producing mutants. / p87 (0097.jp2)
  40. 5.5.Conclusion. / p89 (0099.jp2)
  41. Chapter6.Hydrocarbon utilisation pathways and their regulation. / p94 (0104.jp2)
  42. 6.1.Detection of cis-benzene glycol as the first benzene metabolite. / p94 (0104.jp2)
  43. 6.2.Induction of the different pathways. / p96 (0106.jp2)
  44. 6.3.A constitutive meta pathway. / p98 (0108.jp2)
  45. 6.4.Effect of benzoate on mutant strains. / p99 (0109.jp2)
  46. 6.5.Indole oxidation. / p107 (0117.jp2)
  47. 6.6.Phenol generation. / p109 (0119.jp2)
  48. 6.7.Conclusions. / p110 (0120.jp2)
  49. Part2.Towards a cis-benzene glycol production process. / p114 (0124.jp2)
  50. Chapter7.Factors affecting cis-benzene glycol production. / p114 (0124.jp2)
  51. 7.1.Choice of carbon source for a production process. / p114 (0124.jp2)
  52. 7.2.Effect of carbon sources concentration. / p116 (0126.jp2)
  53. 7.3.Growth rates of the mutant strain with different media and carbon sources. / p120 (0130.jp2)
  54. 7.4.Effect of iron on the cis-benzene glycol production. / p123 (0133.jp2)
  55. 7.5.Effect of Nitrogen source on cBG production. / p126 (0136.jp2)
  56. 7.7.Optimum pH. / p126 (0136.jp2)
  57. 7.7.Effect of temperature on growth and production. / p129 (0139.jp2)
  58. 7.8.Conclusions. / p132 (0142.jp2)
  59. Chapter8.Effect of benzene concentration. / p133 (0143.jp2)
  60. 8.1.Effect on the growth of the wild type. / p133 (0143.jp2)
  61. 8.2.Benzene concentration influence on indole oxidation. / p134 (0144.jp2)
  62. 8.3.Apparatus and growth methods. / p136 (0146.jp2)
  63. 8.4.Influence of benzene concentration on the initial productivity of the strain JI104-16. / p138 (0148.jp2)
  64. 8.5.Evolution of the productivity during the fermentation. / p140 (0150.jp2)
  65. 8.6.Oxygen demand during exposure to benzene. / p143 (0153.jp2)
  66. 8.8.Conclusions. / p148 (0158.jp2)
  67. Chapter9.Benzene toxicity. / p149 (0159.jp2)
  68. 9.1.Observed effects and possible mechanisms. / p149 (0159.jp2)
  69. 9.2.Benzene interaction with the membrane. / p151 (0161.jp2)
  70. 9.3.Adsorption to the cells macromolecules,DNA,RNA,and proteins. / p152 (0162.jp2)
  71. 9.4.Estimation of the stability of the benzene dioxygenase system. / p153 (0163.jp2)
  72. 9.5.Inactivation of the reaction site by benzene metabolites. / p158 (0168.jp2)
  73. 9.6.Inactivation of the active site by oxygen radicals. / p163 (0173.jp2)
  74. 9.7.cis-Benzene glycol production under a reduced oxygen concentration. / p166 (0176.jp2)
  75. 9.8.Conclusions. / p169 (0179.jp2)
  76. Chapter10.Perspectives towards a production process. / p170 (0180.jp2)
  77. 10.1.Outline of a process. / p170 (0180.jp2)
  78. 10.2.Benzene transformation to cis-benzene glycol. / p171 (0181.jp2)
  79. 10.3.Product recovery. / p173 (0183.jp2)
  80. 10.4.Perspectives for further studies. / p174 (0184.jp2)
  81. Summary and concluding remarks. / p176 (0186.jp2)
  82. Bibliography. / p181 (0192.jp2)
  83. Annexes. / p208 (0219.jp2)
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各種コード

  • NII論文ID(NAID)
    500000098149
  • NII著者ID(NRID)
    • 8000000098378
  • DOI(NDL)
  • NDL書誌ID
    • 000000262463
  • データ提供元
    • NDL ONLINE
    • NDLデジタルコレクション

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