Identification, characterization and purification of spider toxin binding protein クモ毒結合蛋白質の精製、ならびに性状解析
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著者
書誌事項
- タイトル
-
Identification, characterization and purification of spider toxin binding protein
- タイトル別名
-
クモ毒結合蛋白質の精製、ならびに性状解析
- 著者名
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Hossain, Muhammed Anwar
- 著者別名
-
ホサイン, マホメッド アノワル
- 学位授与大学
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東京大学
- 取得学位
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博士 (薬学)
- 学位授与番号
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甲第9267号
- 学位授与年月日
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1991-12-11
注記・抄録
博士論文
目次
- Abstract / p1 (0006.jp2)
- Contents / (0009.jp2)
- Introduction / p4 (0015.jp2)
- Materials and Methods / p12 (0023.jp2)
- A. Materials / p13 (0024.jp2)
- B. Methods / p14 (0025.jp2)
- i.Preparation of P-2 fraction / p14 (0025.jp2)
- ii.Solubilization of P-2 fraction protein with detergent / p15 (0026.jp2)
- iii.Production of antibodies / p15 (0026.jp2)
- iv.Purification of antibodies / p16 (0027.jp2)
- v.Electrophysiological studies / p16 (0027.jp2)
- vi.Determination of intracellular Ca²⁺ concentration in primary culture of rat hippocampal neurones / p17 (0028.jp2)
- vii.SDS-Polyacrylamide gel electrophoresis / p18 (0029.jp2)
- viii.Western blot analysis / p18 (0029.jp2)
- ix.Chromatographic methods / p20 (0031.jp2)
- x. N-terminal sequence analysis / p24 (0035.jp2)
- xi.Amino acid composition analysis / p24 (0035.jp2)
- xii.Determination of isoelectricpoint of 60kDa spider toxin binding protein / p24 (0035.jp2)
- xiii.Binding assay / p24 (0035.jp2)
- xiv.Protein measurement / p25 (0036.jp2)
- Results / p26 (0037.jp2)
- i.Solubilization of JSTX-3 binding protein from bovine brain / p27 (0038.jp2)
- ii.Development of antibodies against 1-NA-Spm binding protein / p30 (0041.jp2)
- iii.Effect of antibodies on excitatory postsynaptic potentials / p32 (0043.jp2)
- iv.Effect of Ab-30 on glutamate induced increment of intracellular Ca²⁺ concentration / p35 (0046.jp2)
- v.Immunodetection of 60kDa spider toxin binding protein / p35 (0046.jp2)
- vi.Purification of 60kDa spider toxin binding protein from bovine brain / p38 (0049.jp2)
- vii.Determination of some biophysical and chemical properties of HPLC purified spider toxin binding protein / p45 (0056.jp2)
- Discussions / p56 (0067.jp2)
- References / p66 (0010.jp2)