Pseudomonas fragiにおける脂肪酸β酸化系に関する研究
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著者
書誌事項
- タイトル
-
Pseudomonas fragiにおける脂肪酸β酸化系に関する研究
- 著者名
-
佐藤, 恵春
- 著者別名
-
サトウ, シゲハル
- 学位授与大学
-
東京大学
- 取得学位
-
博士 (理学)
- 学位授与番号
-
甲第9424号
- 学位授与年月日
-
1992-03-30
注記・抄録
博士論文
目次
- CONTENTS / p2 (0004.jp2)
- ACKNOWLEDGEMENTS / p1 (0003.jp2)
- CONTENTS / p2 (0004.jp2)
- ABBREVIATIONS / p5 (0007.jp2)
- SUMMARY / p1 (0008.jp2)
- INTRODUCTION / p4 (0011.jp2)
- MATERIALS AND METHODS / p8 (0015.jp2)
- 1.Bacteria,Phages,and Plasmids / p8 (0015.jp2)
- 2.Materials / p8 (0015.jp2)
- 3.Media and Growth Conditions / p10 (0017.jp2)
- 4.Transformation / p10 (0017.jp2)
- 5.Preparation of Cell Extracts / p11 (0018.jp2)
- 6.Assay for Activities of Enzymes Involved in Fatty Acid β-Oxidation / p12 (0019.jp2)
- 7.Preparation of Antisera Against the Subunits of HDT / p14 (0021.jp2)
- 8.Western Blotting / p14 (0021.jp2)
- 9.Extraction of Genomic DNA from P.fragi Cells / p15 (0022.jp2)
- 10.Construction of Genomic DNA Libraries and Screening / p16 (0023.jp2)
- 11.DNA Sequencing / p17 (0024.jp2)
- 12.Extraction of Total RNA from P.fragi Cells / p18 (0025.jp2)
- 13.Northern Blotting / p19 (0026.jp2)
- 14.Primer Extension Analysis / p19 (0026.jp2)
- 15.Construction of the Plasmids for Promoter Analysis ... / p20 (0027.jp2)
- 16.Assay for β-Galactosidase Activity / p21 (0028.jp2)
- RESULTS / p22 (0029.jp2)
- Section I.Induction of Enzymes Involved in Fatty Acid β-0xidation by Fatty Acids and Their Derivatives / p22 (0029.jp2)
- 1.Changes in the activities of ACDH and HDT during the course of cell growth with or without palmitic acid / p22 (0029.jp2)
- 2.Effects of fatty acids on the inductions of the ACDH and HDT activities / p23 (0030.jp2)
- 3.Effects of fatty acid derivatives on the inductions of the ACDH and HDT activities / p23 (0030.jp2)
- Section II.Primary Structure of HDT,and the Regions Exhibiting Each Component Enzyme Activities / p25 (0032.jp2)
- 1.Priparation of antisera against HDT / p25 (0032.jp2)
- 2.Molecular cloning of the faoA and faoB genes / p25 (0032.jp2)
- 3.Expression of the faoA and/or faoB gene products and their enzyme activities in E. coli / p27 (0034.jp2)
- Section III.Expression of HDT and its Regulation in P.fragi / p29 (0036.jp2)
- 1.Changes in the amounts of HDT protein during the course of cell growth with palmitic acid / p29 (0036.jp2)
- 2.Transcription of the mRNA encoding the genes for HDT / p29 (0036.jp2)
- 3.Effect of the 5' upstream region of faoA on the expression of faoA gene / p31 (0038.jp2)
- DISCUSSION / p33 (0040.jp2)
- I.Primary Structure of HDT,and the Regions Exhibiting Each Enzyme Activities / p33 (0040.jp2)
- II.Expression of the Fatty Acid β-Oxidation Enzymes and its Regulation / p38 (0045.jp2)
- CONCLUSION / p44 (0051.jp2)
- REFERENCES / p46 (0053.jp2)
- TABLES / p51 (0058.jp2)
- FIGURES / p54 (0061.jp2)