大腸菌シトクロムbo複合体の酸化還元反応中心の構造

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TABLE OF CONTENTS / (0010.jp2)
Chapter I.General Introduction / p1 (0013.jp2)
Background / p2 (0014.jp2)
Scope of this thesis / p9 (0021.jp2)
Table / p10 (0022.jp2)
Figures / p11 (0023.jp2)
Chapter II.Experimental Procedures / p19 (0031.jp2)
Media / p20 (0032.jp2)
Bacterial Strains / p20 (0032.jp2)
Genetic Procedures and DNA Manipulations / p21 (0033.jp2)
Introduction of additional restriction sites in the cyoB gene / p22 (0034.jp2)
Site-directed Mutagenesis / p23 (0035.jp2)
Preparation of Cytoplasmic Membranes and Purification of Mutant Enzymes / p25 (0037.jp2)
Complementation Test for Aerobic Growth / p25 (0037.jp2)
Optical Spectroscopy / p26 (0038.jp2)
EPR Spectroscopy / p27 (0039.jp2)
Other methods / p27 (0039.jp2)
Materials / p28 (0040.jp2)
Tables / p29 (0041.jp2)
Figures / p32 (0044.jp2)
Chapter III.Replacements of the Conserved His Residues / p35 (0047.jp2)
Introduction / p36 (0048.jp2)
Results / p42 (0054.jp2)
Discussion / p50 (0062.jp2)
Tables / p57 (0069.jp2)
Figures / p63 (0075.jp2)
Chapter IV.Replacements of Conserved Aromatic Amino Acid Residues / p72 (0084.jp2)
Introduction / p73 (0085.jp2)
Results / p76 (0088.jp2)
Discussion / p82 (0094.jp2)
Table / p88 (0100.jp2)
Figures / p89 (0101.jp2)
Chapter V.Conclusion / p99 (0111.jp2)
REFERENCES / p104 (0116.jp2)