Transcriptional regulation of the POU-M1/SGF-3 gene involved in the expression of Bombyx silk genes Transcriptional Regulation of the POU-M1/SGF-3 Gene Involved in the Expression of Bombyx Silk Genes

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Title: Transcriptional regulation of the POU-M1/SGF-3 gene involved in the expression of Bombyx silk genes

Other Title: Transcriptional Regulation of the POU-M1/SGF-3 Gene Involved in the Expression of Bombyx Silk Genes

博士論文

総研大甲第54号

CONTENTS / p2 (0003.jp2)
1.Abstract / p4 (0005.jp2)
2.Introduction / p5 (0006.jp2)
3.Experimental Procedures / p8 (0009.jp2)
Isolation of Genomic and cDNA Clones / p8 (0009.jp2)
Southern Blotting / p8 (0009.jp2)
DNA Sequencing / p9 (0010.jp2)
S1 Mapping / p9 (0010.jp2)
Primer Extension / p10 (0011.jp2)
Preparation of Nuclear Extracts / p10 (0011.jp2)
Construction of Deletion Mutants / p10 (0011.jp2)
DNase I Footprinting and Gel Retardation Analysis / p11 (0012.jp2)
Hyper-Shift Assay / p12 (0013.jp2)
In vitro Transcription Analysis of POU-M1/SGF-3 Promoter / p12 (0013.jp2)
Immunodepletion / p13 (0014.jp2)
4.Results / p14 (0015.jp2)
Structure of the POU-M1/SGF-3 Gene / p14 (0015.jp2)
Localization of the 5'-End of the POU-M1/SGF-3 Gene / p15 (0016.jp2)
DNA Sequence of the 5'-Flanking Region of the POU-M1/SGF-3 Gene / p15 (0016.jp2)
DNase I Footprint Analysis of the Presumed Promoter Region / p16 (0017.jp2)
Binding of the POU-M1/SGF-3 Factor to the PB Region / p17 (0018.jp2)
Gel Shift Analysis of the PA,PC and Possible Sp1-binding Sites / p19 (0020.jp2)
Analysis of POU-M1/SGF-3 cis-Acting Control Elements / p20 (0021.jp2)
Immunodepletion of POU-M1/SGF-3 Results in Stimulation of the POU-M1/SGF-3 Transcription / p22 (0023.jp2)
5.Discussion / p23 (0024.jp2)
6.References / p28 (0029.jp2)
7.Acknowledgments / p34 (0035.jp2)
8.Figures / p35 (0036.jp2)
9.List of Publication / p44 (0057.jp2)

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