The development and the practical appreciation of neural cell-type-specific expression system with replication-defective adenovirus vectors アデノウイルスベクターを用いた神経細胞型特異的遺伝子導入法の開発と応用
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Bibliographic Information
- Title
-
The development and the practical appreciation of neural cell-type-specific expression system with replication-defective adenovirus vectors
- Other Title
-
アデノウイルスベクターを用いた神経細胞型特異的遺伝子導入法の開発と応用
- Author
-
橋本, 光広
- Author(Another name)
-
ハシモト, ミツヒロ
- University
-
大阪大学
- Types of degree
-
博士 (理学)
- Grant ID
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甲第5432号
- Degree year
-
1996-03-25
Note and Description
博士論文
Table of Contents
- <CONTENTS> / (0003.jp2)
- CHAPTER I GENERAL INTRODUCTION / 1-1 / (0008.jp2)
- SECTION 1 INTRODUCTION OF VIRAL VECTORS / 1-1 / (0008.jp2)
- SECTION 2 ADENOVIRUS BIOLOGY / 1-5 / (0012.jp2)
- Fig.I-1 / Fig.1-1 / (0014.jp2)
- The properties of animal DNA viruses (deoxyribovirus). / (0015.jp2)
- Fig.I-2 / Fig.1-2 / (0016.jp2)
- The properties of human adenovirus serotype 5. / (0017.jp2)
- Fig.I-3 / Fig.1-3 / (0018.jp2)
- Transcription and translation map of adenovirus. / (0019.jp2)
- Fig.I-4 / Fig.1-4 / (0020.jp2)
- The life cycle of wild type adenovirus, replication defective adenovirus vectors and replication-defective adenovirus vectors in 293 cells. / (0021.jp2)
- Fig.I-5 / Fig.1-5 / (0022.jp2)
- The stepwise transport of adenoviruses from a cell surface to a nucleus. / (0023.jp2)
- CHAPTER II MATERIALS AND METHODS / 2-1 / (0024.jp2)
- A.Viral genome construction / 2-1 / (0024.jp2)
- B.Preparation of recombinant adenovirus / 2-4 / (0027.jp2)
- C.Cell culture / 2-5 / (0028.jp2)
- D.Serum-free primary culture of mouse cerebellum / 2-6 / (0029.jp2)
- E.Serum-free primary culture of oligodendrocyte / 2-7 / (0030.jp2)
- F.Primary culture of astrocytes from rat striatum / 2-7 / (0030.jp2)
- G.Slice culture of cerebellum / 2-8 / (0031.jp2)
- H.In vivo infection with adenovirus / 2-9 / (0032.jp2)
- I.β-galactosidase cytochemistry / 2-10 / (0033.jp2)
- J.Immunohistochemistry / 2-10 / (0033.jp2)
- Fig.II-l / Fig.2-1 / (0035.jp2)
- Constructs of recombinant adenoviruses. / (0036.jp2)
- Fig.II-2 / Fig.2-2 / (0037.jp2)
- Constructs of AdexGFAP-NL-LacZ and AdexGFAPhTHl. / (0038.jp2)
- Fig.II-3 / Fig.2-3 / (0039.jp2)
- In vivo injection with a stereotaxic technique. / (0040.jp2)
- CHAPTER III A NEURAL CELL-TYPE SPECIFIC EXPRESSION SYSTEM USING RECOMBINANT ADENOVIRUS VECTORS / 3-1 / (0041.jp2)
- INTRODUCTION / 3-1 / (0041.jp2)
- RESULTS / 3-4 / (0044.jp2)
- DISCUSSION / 3-12 / (0052.jp2)
- ACKNOWLEDGEMENTS / 3-17 / (0057.jp2)
- Fig.III-1 / Fig.3-1 / (0058.jp2)
- Constructs of recombinant adenoviruses. / (0059.jp2)
- Fig.III-2 / Fig.3-2 / (0060.jp2)
- The β-galactosidase histochemical staining of mouse cerebellar cells in primary culture. / (0061.jp2)
- Fig.III-3 / Fig.3-2 / (0062.jp2)
- Oligodendrocyte-specific expression with AdexMBP-NL-LacZ in the serum-free primary culture of oligodendrocyte. / (0063.jp2)
- Fig.III-4 / Fig.3-4 / (0064.jp2)
- Expression of β-galactosidase activity in vivo following an injection of AdexCAG-NL-LacZ or Adex△P-NL-LacZ, into rat cerebella. / (0065.jp2)
- Fig.III-5 / Fig.3-5 / (0066.jp2)
- Purkinje cell specific gene expression by AdexL7-NL-LacZ. / (0067.jp2)
- Fig.III-6 / Fig.3-6 / (0068.jp2)
- Oligodendrocyte-specific gene expression using AdexMBP-NL-LacZ. / (0069.jp2)
- CHAPTER IV CONSTRUCTION OF GLIAL CELL-TYPE-SPECIFIC ADENOVIRUS VECTORS AND GENETICAL MODIFICATION OF PRIMARY ASTROCYTES TO EXPRESS TYROSINE HYDROXYLASE / 4-1 / (0070.jp2)
- INTRODUCTION / 4-1 / (0070.jp2)
- RESULTS / 4-4 / (0073.jp2)
- DISCUSSION / 4-8 / (0077.jp2)
- ACKNOWLEDGEMENT / 4-13 / (0082.jp2)
- Fig.IV-1 / Fig.4-1 / (0083.jp2)
- Dopamine biosynthetic pathway. / (0084.jp2)
- Fig.IV-2 / Fig.4-2 / (0085.jp2)
- Constructs of AdexGFAP-NL-LacZ and AdexGFAPhThl. / (0086.jp2)
- Fig.IV-3 / Fig.4-3 / (0087.jp2)
- In vitro glial cell-specific expression with AdexGFAP-NL-LacZ. / (0088.jp2)
- Fig.IV-4 / Fig.4-4 / (0089.jp2)
- In vivo glial cell-specific gene expression using AdexGFAP-NL-LacZ. / (0090.jp2)
- Fig.IV-5 / Fig.4-5 / (0091.jp2)
- Expression of human tyrosine hydroxylase type-1 in C6 glioma cells infected with AdexGFAPhTHl. / (0092.jp2)
- Fig.IV-6 / Fig.4-6 / (0093.jp2)
- Expression of human tyrosine hydroxylase type-1 in a primary culture of astrocytes with AdexGFAPhTHl. / (0094.jp2)
- CHAPTER V ADENOVIRUS-MEDIATED GENE EXPRESSION IN THE ORGANOTYPIC SLICE CULTURE OF RAT CEREBELLUM / 5-1 / (0095.jp2)
- INTRODUCTION / 5-1 / (0095.jp2)
- RESULTS / 5-2 / (0096.jp2)
- DISCUSSION / 5-3 / (0097.jp2)
- Fig.V-1 / Fig.5-1 / (0098.jp2)
- Adenovirus-mediated gene expression in the organotypic slice culture of rat cerebellum. / (0099.jp2)
- REFERENCES / REFERENCES-1 / (0100.jp2)